2-D Proteome Analysis Protocols

  • Andrew J. Link

Part of the Methods in Molecular Biology book series (MIMB, volume 112)

Table of contents

  1. Front Matter
    Pages i-xvii
  2. Jenny Fichmann, Reiner Westermeier
    Pages 1-7
  3. Ruth A. VanBogelen, Frederick C. Neidhardt
    Pages 21-29
  4. David M. Schieltz
    Pages 31-34
  5. Melinda L. Ramsby, Gregory S. Makowski
    Pages 53-66
  6. Jean-Charles Sanchez, Denis F. Hochstrasser
    Pages 87-93
  7. Akira Tsugita, Masaharu Kamo
    Pages 95-97
  8. Andrew J. Link, Nick Bizios
    Pages 105-107
  9. Joachim Klose
    Pages 147-172
  10. Jenny Fichmann
    Pages 173-174
  11. Elisabetta Gianazza
    Pages 175-188
  12. Angelika Görg, Walter Weiss
    Pages 189-195
  13. Angelika Görg
    Pages 197-209
  14. Neil M. Matsui, Diana M. Smith-Beckerman, Jenny Fichmann, Lois B. Epstein
    Pages 211-219
  15. Jean-Charles Sanchez, Denis Hochstrasser, Thierry Rabilloud
    Pages 221-225
  16. Jean-Charles Sanchez, Denis F. Hochstrasser
    Pages 227-233
  17. Angelika Görg, Walter Weiss
    Pages 235-244
  18. Bradley J. Walsh, Benjamin R. Herbert
    Pages 245-253
  19. Hong Ji, Richard J. Simpson
    Pages 255-263
  20. Joan Goverman
    Pages 265-270
  21. Hikaru Nagahara, Robert R. Latek, Sergei A. Ezhevsky, Steven F. Dowdy
    Pages 271-279
  22. Andrew J. Link
    Pages 281-284
  23. Andrew J. Link
    Pages 285-290
  24. Kelvin H. Lee, Michael G. Harrington
    Pages 291-295
  25. Thierry Rabilloud
    Pages 297-305
  26. Neil M. Matsui, Diana M Smith-Beckerman, Lois B. Epstein
    Pages 307-311
  27. Wayne F. Patton, Mark J. Lim, David Shepro
    Pages 331-339
  28. Nicolle H. Packer, Malcolm S. Ball, Peter L. Devine
    Pages 341-352
  29. Wayne F. Patton, Mark J. Lim, David Shepro
    Pages 353-362
  30. Ron D. Appel, Denis F. Hochstrasser
    Pages 363-381
  31. Ron D. Appel, Amos Bairoch, Denis F. Hochstrasser
    Pages 383-391
  32. Ron D. Appel, Christine Hoogland, Amos Bairoch, Denis F. Hochstrasser
    Pages 411-416
  33. Steven P. Gygi, Ruedi Aebersold
    Pages 417-421
  34. Ruth A. VanBogelen
    Pages 423-429
  35. Barbara Magi, Luca Bini, Barbara Marzocchi, Sabrina Liberatori, Roberto Raggiaschi, Vitaliano Pallini
    Pages 431-443
  36. Marc R. Wilkins, Jun X. Yan, Andrew A. Gooley
    Pages 445-460
  37. Masaharu Kamo, Akira Tsugita
    Pages 461-466

About this book


In 2-D Gel Proteome Analysis Protocols, Andrew Link and his expert collaborators take today's researchers step-by-step through the complete process of doing proteomics. With easy-to-follow instructions, complete with many helpful hints and explanations, leading investigators and pioneers in the field show how to make protein extracts, reproducibly run them on a 2-D gels, detect them, analyze the data, and precisely identify each protein. The book covers the latest methods of using carrier ampholytes in the 1st dimension, casting and running immobilized pH gradient 2-D gels, MALDI-TOF-based peptide mapping, automated tandem mass spectrometry, and nanoelectrospray ionization technology. For the 2nd dimension, there are methods for running flatbed or vertical gels and for protein detection using autoradiography, and Coomassie, silver, and reversible metal-chelate stains. The book is a perfect complement to the genome sequencing project for answering biological questions.

2-D Gel Proteome Analysis Protocols is the most complete guide for using proteomics to answer biological questions. Whether it is a question of global protein analysis or evaluating a cell's response to internal or external stimuli, the advanced methods described here will enable today's researchers better to understand how cells work and open new possibilities for drug discovery.

Editors and affiliations

  • Andrew J. Link
    • 1
  1. 1.Department of Molecular BiotechnologyUniversity of WashingtonSeattle

Bibliographic information

  • DOI
  • Copyright Information Humana Press 1999
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-524-9
  • Online ISBN 978-1-59259-584-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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