Plant Cell Culture Protocols

  • Robert D. Hall

Part of the Methods In Molecular Biology™ book series (MIMB, volume 111)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Introduction

    1. Front Matter
      Pages N1-N1
    2. Robert D. Hall
      Pages 1-18
  3. Cell Culture and Plant Regeneration

    1. Front Matter
      Pages N3-N3
    2. Nigel W. Blackhall, Joan P. Jotham, Kasimalal Azhakanandam, J. Brian Power, Kenneth C. Lowe, Edward C. Cocking et al.
      Pages 19-29
    3. Rosario F. Curry, Alan C. Cassells
      Pages 31-42
    4. Makoto Kihara, Hideyuki Funatsuki, Kazutoshi Ito, Paul A. Lazzeri
      Pages 43-49
    5. Ulrika Egertsdotter
      Pages 51-60
    6. Specialized Techniques

      1. Krit J. J. M. Raemakers, Evert Jacobsen, Richard G. F. Visser
        Pages 61-70
      2. Sonriza Rasco-Gaunt, Pilar Barcelo
        Pages 71-81
      3. Erica E. Benson, Paul T. Lynch
        Pages 83-94
      4. Ali Golmirzaie, Judith Toledo
        Pages 95-101
  4. Plant Propagation In Vitro

    1. Front Matter
      Pages N5-N5
    2. Specialized Techniques

      1. Brent Tisserat, Daniel Jones
        Pages 127-134
      2. Jill Gratton, Michael F. Fay
        Pages 135-140
      3. Merel M. Langens-Gerrits, Geert-Jan M. De Klerk
        Pages 141-147
      4. Indra S. Harry, Trevor A. Thorpe
        Pages 149-157
      5. Matthew V. Ford, Michael F. Fay
        Pages 159-168
  5. Applications for Plant Protoplasts

    1. Front Matter
      Pages N7-N7
    2. Paul Anthony, Wagner Otoni, J. Brian Power, Kenneth C. Lowe, Michael R. Davey
      Pages 169-181
    3. Jan Fahleson, Kristina Glimelius
      Pages 195-210
    4. Specialized Techniques

      1. Kamisetti S. Ramulu, Paul Dijkhuis, Jan Blaas, Frans A. Krens, Harrie A. Verhoeven
        Pages 227-242
      2. Graham Boorse, Gary Tallman
        Pages 243-257
  6. Protocols for Genomic Manipulation

    1. Front Matter
      Pages N9-N9
    2. Alwine Jähne-Gärtner, Horst Lörz
      Pages 269-279
    3. Alisher Touraev, Erwin Heberle-Bors
      Pages 281-291
    4. Hari C. Sharma
      Pages 293-307
    5. Specialized Techniques

  7. Protocols for the Introduction of Specific Genes

    1. Front Matter
      Pages N11-N11
    2. Karabi Datta, Swapan K. Datta
      Pages 335-347
    3. Indra K. Vasil, Vimla Vasil
      Pages 349-358
    4. Specialized Techniques

      1. Kathleen D’Halluin, Els Bonne, Martien Bossut, Rosita Le Page
        Pages 367-373
  8. Suspension Culture Initiation and the Accumulation of Metabolites

    1. Front Matter
      Pages N13-N13
    2. Marc J. M. Hagendoorn, Diaan C. L. Jamar, Linus H. W. van der Plas
      Pages 383-392
    3. Christopher S. Hunter, Nigel J. Kilby
      Pages 403-410
  9. Back Matter
    Pages 411-421

About this book


In Plant Cell Culture Protocols, Robert Hall and a panel of expert researchers present a comprehensive collection of the most frequently used and broadly applicable techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods cover culture initiation, maintenance, manipulation, application, and long-term storage, with emphasis on techniques for genetic modification and micropropagation. Many of these protocols are currently used in major projects designed to produce improved varieties of important crop plants. In addition, a number of specialized protocols have been included to illustrate the diversity of the techniques available and their widespread applicability.
Plant Cell Culture Protocols is aimed at scientists involved in all aspects of plant biotechnological research, as well as those working in other areas of agriculture and horticulture who are interested in expanding their technical repertoire to include in vitro methodology. Its state-of-the-art techniques are certain to make the book today's reference of choice, an indispensable tool in the development of new transgenic plants and full-scale commercial applications.

Editors and affiliations

  • Robert D. Hall
    • 1
  1. 1.CPRO-DLOWageningenThe Netherlands

Bibliographic information

  • DOI
  • Copyright Information Humana Press 1999
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-549-2
  • Online ISBN 978-1-59259-583-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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