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E. coli Plasmid Vectors

Methods and Applications

  • Book
  • © 2003

Overview

Editors:
  1. Nicola Casali

    1. University of California at Berkeley, School of Public Health, Berkeley

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  2. Andrew Preston

    1. Department of Clinical Veterinary Medicine, University of Cambridge, Cambridge, UK

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    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 235)

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Table of contents (31 protocols)

  1. Restriction Analysis of Recombinant Plasmids

    • Joanne Goranson-Siekierke, Jarrod L. Erbe
    Pages 175-181

  2. Screening Recombinant DNA Libraries

    • Wade A. Nichols
    Pages 183-194

  3. Sequencing Using Fluorescent-Labeled Nucleotides

    • Allison F. Gillaspy
    Pages 195-202

  4. Site-Directed Mutagenesis Using the Megaprimer Method

    • Zhidong Xu, Alessia Colosimo, Dieter C. Gruenert
    Pages 203-207

  5. Site-Directed Mutagenesis by Inverse PCR

    • Clifford N. Dominy, David W. Andrews
    Pages 209-223

  6. Creating Nested DNA Deletions Using Exonuclease III

    • Rosamund Powles, Lafras M. Steyn
    Pages 225-231

  7. Transposon and Transposome Mutagenesis of Plasmids, Cosmids, and BACs

    • Alistair McGregor
    Pages 233-245

  8. In Vitro Transcription and Translation

    • Farahnaz Movahedzadeh, Susana González Rico, Robert A. Cox
    Pages 247-255

  9. Vectors for the Expression of Recombinant Proteins in E. coli

    • Sally A. Cantrell
    Pages 257-275

  10. Expression of Recombinant Proteins From lac Promoters

    • Charles R. Sweet
    Pages 277-288

  11. Plasmid-Based Reporter Genes

    • Minghsun Liu
    Pages 289-296

  12. Plasmid-Based Reporter Genes

    • Sergei R. Doulatov
    Pages 297-304

  13. Back Matter

    Pages 305-316
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Keywords

About this book

A comprehensive collection of readily reproducible techniques for the manipulation of recombinant plasmids using the bacterial host E. coli. The authors describe proven methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones. They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, l vectors, and phagemids. Common downstream applications such as mutagenesis of plasmids, recombinant protein expression, and the use of reporter genes, are also described.

Reviews

...a scientific review and a methodological "cook-book" of high quality. It is truly a highly practical handbook, useful for all scientists working with gene cloning and manipulation." - Folia Microbiologica

"...a very useful addition to laboratories investigating plasmids per se, and for those employing plasmids as vectors for gene cloning." - Microbiology Today

Editors and Affiliations

  • University of California at Berkeley, School of Public Health, Berkeley

    Nicola Casali

  • Department of Clinical Veterinary Medicine, University of Cambridge, Cambridge, UK

    Andrew Preston

Bibliographic Information

  • Book Title: E. coli Plasmid Vectors

  • Book Subtitle: Methods and Applications

  • Editors: Nicola Casali, Andrew Preston

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592594093

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2003

  • Hardcover ISBN: 978-1-58829-151-6Published: 03 July 2003

  • Softcover ISBN: 978-1-61737-391-6Published: 10 November 2010

  • eBook ISBN: 978-1-59259-409-2Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 316

  • Topics: Biochemistry, general, Cell Biology

  • Industry Sectors: Biotechnology, Consumer Packaged Goods, Pharma

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