Table of contents

  1. Front Matter
    Pages i-xi
  2. Andrew Preston
    Pages 19-26
  3. Nicola Casali
    Pages 27-48
  4. W. Edward Swords
    Pages 49-53
  5. Claire A. Woodall
    Pages 55-59
  6. Claire A. Woodall
    Pages 61-65
  7. Mallory J. A. White, Wade A. Nichols
    Pages 67-73
  8. Sabine Ehrt, Dirk Schnappinger
    Pages 75-78
  9. Sabine Ehrt, Dirk Schnappinger
    Pages 79-82
  10. Stefan Grimm, Frank Voß-Neudecker
    Pages 83-87
  11. Daniel Sinnett, Alexandre Montpetit
    Pages 99-102
  12. Carey Pashley, Sharon Kendall
    Pages 121-135
  13. Nicholas Downey
    Pages 137-139
  14. Wade A. Nichols
    Pages 141-152
  15. Yilun Wang, Zheng Cao, Darryl Hood, James G. Townsel
    Pages 153-168
  16. Sangwei Lu
    Pages 169-174

About this book

Introduction

Manipulation of recombinant DNA, which is almost exclusively performed using the host E. coli, constitutes one of the fundamental methodologies of molecular biotechnology. In E. coli Plasmid Vectors, experienced bench researchers describe their proven techniques for the manipulation of recombinant plasmids utilizing this popular bacterial host. The authors describe readily reproducible methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones. They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, l vectors, and phagemids. Also presented are methods for common downstream applications, such as mutagenesis, expression of recombinant proteins and RNA transcripts, and uses of reporter genes. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, tips on troubleshooting and avoiding known pitfalls and, where needed, a discussion of the interpretation and use of the results.
Comprehensive and highly practical, E. coli Plasmid Vectors offers those new to the field a basic guide to the use of plasmid vectors in the cloning host E. coli, and those more experienced researchers a broad-ranging, proven array of successful techniques.

Keywords

BAC DNA Escherichia coli PCR Promoter Translation chromosome genes reproducible techniques transcription

Editors and affiliations

  • Nicola Casali
    • 1
  • Andrew Preston
    • 2
  1. 1.University of California at BerkeleySchool of Public HealthBerkeley
  2. 2.Department of Clinical Veterinary MedicineUniversity of CambridgeCambridgeUK

Bibliographic information

  • DOI https://doi.org/10.1385/1592594093
  • Copyright Information Humana Press 2003
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-151-6
  • Online ISBN 978-1-59259-409-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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