Cancer Cell Culture

Methods and Protocols

  • Simon P. Langdon

Part of the Methods in Molecular Medicine™ book series (MIMM, volume 88)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Introduction to Cancer Cell Culture

    1. Front Matter
      Pages 1-1
    2. Simon P. Langdon
      Pages 3-15
    3. Kenneth G. Macleod, Simon P. Langdon
      Pages 17-29
  3. Characterization and Authentication

    1. Front Matter
      Pages 31-31
    2. Wilhelm G. Dirks, Hans G. Drexler
      Pages 43-55
    3. Roderick A. F. MacLeod, Hans G. Drexler
      Pages 57-76
  4. Isolation and Culture of Specialized Cell Types

    1. Front Matter
      Pages 77-77
    2. Jae-Gahb Park, Ja-Lok Ku, So-Yeon Park
      Pages 79-92
    3. German G. Gomez, Carol A. Kruse
      Pages 101-109
    4. Jae-Gahb Park, Ja-Lok Ku, So-Yeon Park
      Pages 111-119
    5. Nora M. Navone, Matilde Olive, Patricia Troncoso
      Pages 121-132
    6. Hans G. Drexler
      Pages 141-155
  5. Functional Assays in Cell Culture

    1. Front Matter
      Pages 157-157
    2. Jane A. Plumb
      Pages 159-164
    3. Jane A. Plumb
      Pages 165-169

About this book

Introduction

The growing of cancer cells in the laboratory has been a powerful tool for studying and understanding the biology of cancer and the effects of drugs on cancer cells. In Cancer Cell Culture: Methods and Protocols, expert researchers describe in detail their most productive and up-to-date methods for growing cancer cells in the laboratory. Suitable for novice and experienced researchers alike, these readily reproducible techniques solve a diverse range of experimental problems. There are methods to characterize and authenticate cell lines, to isolate and develop specific types of cancer cells, and to develop new cell line models. Functional assays are provided to evaluate clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Other methods permit the modification of cancer cells for transfection, development of drug resistance, immortalization, and transfer in vivo; the coculture of different cell types; and the detection and treatment of contamination. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction explaining the principle behind the technique, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and highly practical, Cancer Cell Culture: Methods and Protocols provides both basic scientists and clinical researchers with a gold-standard collection of advanced techniques for culturing cancer cells effectively in their laboratories.

Editors and affiliations

  • Simon P. Langdon
    • 1
  1. 1.Western General Hospital EdinburghUK

Bibliographic information

  • DOI https://doi.org/10.1385/1592594069
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-079-3
  • Online ISBN 978-1-59259-406-1
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • About this book
Industry Sectors
Biotechnology
Pharma