Inflammation Protocols

  • Paul G. Winyard
  • Derek A. Willoughby

Part of the Methods in Molecular Biology book series (MIMB, volume 225)

Table of contents

  1. Front Matter
    Pages i-xii
  2. In Vitro Systems for Studying Aspects of the Inflammatory Response and Testing Antiinflammatory Drugs

    1. Front Matter
      Pages 1-1
    2. M. Rachel Morris, Sharon Dewitt, Iraj Laffafian, Maurice B. Hallett
      Pages 35-46
    3. Sharon Dewitt, Iraj Laffafian, M. Rachel Morris, Maurice B. Hallett
      Pages 47-59
    4. Maurice B. Hallett, Caroline Cole, Sharon Dewitt
      Pages 61-67
    5. Tom Eirik Mollnes
      Pages 69-75
    6. William D. Shingleton
      Pages 99-106
  3. In Vivo Models of Inflammation

    1. Front Matter
      Pages 107-107
    2. Derek A. Willoughby
      Pages 109-113
    3. Christopher J. Morris
      Pages 115-121
    4. Adrian R. Moore
      Pages 123-128
    5. Miklós Gábor
      Pages 129-137
    6. Nancy L. McCartney-Francis, James Chan, Sharon M. Wahl
      Pages 147-159
    7. Adrian R. Moore
      Pages 175-179
    8. Nicole S. Wayman, Michelle C. McDonald, Prabal K. Chatterjee, Christoph Thiemermann
      Pages 199-208
    9. Prabal K. Chatterjee, Christoph Thiemermann
      Pages 223-237
    10. Isabelle Binet, Kathryn J. Wood
      Pages 239-248
    11. Annette Tomlinson, Mark W. J. Ferguson
      Pages 249-260
  4. Pharmacodynamic Endpoints in Experimental Models and In Clinical Studies in Humans

    1. Front Matter
      Pages 261-261
    2. Daniela Salvemini, Harry Ischiropoulos, Salvatore Cuzzocrea
      Pages 291-303
    3. Claire A. Davies, Sophie A. Rocks, Meg C. O’ Shaughnessy, David Perrett, Paul G. Winyard
      Pages 305-320
    4. Chi-Chung Chan
      Pages 321-328
    5. Robert F. Ritchie, Thomas B. Ledue
      Pages 343-352
    6. Jon B. Catterall, Tim E. Cawston
      Pages 353-364
  5. Back Matter
    Pages 365-378

About this book


Because inflammation is involved in many chronic human diseases, its proper understanding may provide important clues to new therapeutic targets. In Inflammation Protocols, highly skilled experimenters present key techniques for the multidisciplinary study of inflammation in such conditions as inflammatory bowel disease, rheumatoid arthritis, renal disease, and cardiovascular disease. Described in step-by-step detail to ensure experimental success, the techniques are focused on the research and development of potential new antiinflammatory drugs in active target areas. The protocols are suitable for many current areas of drug discovery research, including transcription factors, cytokines, adhesion molecules, cyclooxygenase-2 (COX-2) inhibitors, free radicals, nitric oxide synthases, complement activation, angiogenesis, wound healing, immune rejection, and metalloproteinases. The methods provide in vitro systems for studying inflammation, in vivo models, and relevant pharmacodynamic measurements for the assessment of antiinflammatory compounds. Each protocol contains notes about potential pitfalls and tips on how to avoid failure. Overview chapters at the start of each section review the significance and potential limitations of the protocols presented.
Cutting-edge and highly practical, Inflammation Protocols offers experimental and clinical researchers a premier collection of readily reproducible techniques not only for evaluating inflammatory disease processes, but also for the discovery of new drugs for treating chronic human inflammatory diseases today.

Editors and affiliations

  • Paul G. Winyard
    • 1
  • Derek A. Willoughby
    • 1
  1. 1.William Harvey Research InstituteLondonUK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2003
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-970-4
  • Online ISBN 978-1-59259-374-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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