Hemoglobin Disorders

Molecular Methods and Protocols

  • Ronald L. Nagel

Part of the Methods in Molecular Biology™ book series (MIMM, volume 82)

Table of contents

  1. Front Matter
    Pages i-xx
  2. Martin K. Safo, Donald J. Abraham
    Pages 1-19
  3. Jean Kister, Henri Wajcman
    Pages 49-64
  4. John S. Olson, Erin W. Foley, David H. Maillett, Eden V. Paster
    Pages 65-91
  5. John M. Old
    Pages 101-116
  6. John M. Old
    Pages 117-131
  7. Rhoda Elison Hirstch
    Pages 133-154
  8. Peter G. Vekilov, Angela Feeling-Taylor, Rhoda Elison Hirsch
    Pages 155-176
  9. Seetharama A. Acharya, Sonati Srinivasulu
    Pages 177-193
  10. Shanmugakonar Muralitharan, Rajagopal Krishnamoorthy, Ronald L. Nagel
    Pages 195-211
  11. Mary E. Fabry, Eric E. Bouhassira, Sandra M. Suzuka, Ronald L. Nagel
    Pages 213-241
  12. Jonathan A. Lukin, Chien Ho
    Pages 251-269
  13. Mary E. Fabry, Seetharama A. Acharya, Sandra M. Suzuka, Ronald L. Nagel
    Pages 271-287
  14. Back Matter
    Pages 289-300

About this book


The recent announcement that sickle-cell anemia and thalassemia have been corrected by the transplantation of stem cells bodes well for the future of gene therapy in hemoglobinopathies. In Hemoglobin Disorders: Molecular Methods and Protocols, Ronald Nagel, MD, has assembled a collection of readily reproducible techniques essential to the continued advance of our molecular understanding of these diseases. The book's richly experienced authors detail methods utilizing a wide variety of the latest analytical techniques, including X-ray crystallography, high performance liquid chromatography, electrophoresis, and nuclear magnetic resonance. Additional methods are offered for prenatal diagnostic analysis, the DNA diagnosis of hemoglobin mutations, hemoglobin fluorescence, and the semisynthesis of hemoglobin. Each protocol includes an introduction explaining the basic science, step-by-step instructions for its successful execution, notes on pitfalls to avoid, and tips on how to employ it effectively with novel systems and conditions.
State-of-the-art and highly practical, Hemoglobin Disorders: Molecular Methods and Protocols reviews all the basic topics and techniques in this critically important field, and offers today's most comprehensive set of proven protocols for successful experimental and clinical work on hemoglobin diseases.

Editors and affiliations

  • Ronald L. Nagel
    • 1
  1. 1.Albert Einstein College of MedicineBronx

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2003
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-962-9
  • Online ISBN 978-1-59259-373-6
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • Buy this book on publisher's site
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