Cytokines and Colony Stimulating Factors

Methods and Protocols

  • Dieter Körholz
  • Wieland Kiess

Part of the Methods in Molecular Biology™ book series (MIMB, volume 215)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Introduction

  3. Detection Assays for Cytokines and Growth Factors

    1. Front Matter
      Pages 12-133
    2. Ursula Banning, Dieter Körholz
      Pages 21-22
    3. Jean Krutmann, Renz Mang, Susanne Grether-Beck
      Pages 23-27
    4. Bodo Hoffmeister, Felix Kiecker, Ingolf Surel, Elham Khatamzas, Volker Schuster, Hans-Dieter Volk et al.
      Pages 59-70
    5. Thomas Lehrnbecher, Stephen J. Chanock
      Pages 71-94
    6. Angelika Eggert, Hauke Sieverts, Henning Walczak
      Pages 95-116
    7. Angelika Eggert, John H. Maris
      Pages 117-133
  4. Detection Assays for Cytokines and Growth Factors

    1. Front Matter
      Pages 137-289
    2. Christof M. Kramm, Tim Niehues, Nikolai G. Rainov
      Pages 137-152
    3. Ciriaco A. Piccirillo, Gérald J. Prud’homme
      Pages 153-170
    4. Yuti Chernajovsky, Hanna Dreja, Kostas Triantaphyllopoulos, David Gould
      Pages 171-180
    5. Tohru Sakai, Kunisuke Himeno
      Pages 181-191
    6. Arne von Bonin, Solveig H. Moré, Minka Breloer
      Pages 193-200
    7. Glyn D. Palmer, Elvire Gouze, Jean-Noel Gouze, Oliver B. Betz, Christopher H. Evans, Steven C. Ghivizzani
      Pages 235-246
    8. Salima Hacein-Bey-Abina, Genevieve de Saint Basile, Marina Cavazzana-Calvo
      Pages 247-260
    9. Dagmar Dilloo, Andree Zibert
      Pages 261-277
    10. Roberto Furlan, Stefano Pluchino, Peggy C. Marconi, Gianvito Martino
      Pages 288-289
  5. Ex Vivo Cell Expansion

    1. Front Matter
      Pages 291-395
    2. Bruno Canque, Michele Rosenzwajg, Jean Claude Gluckman
      Pages 321-325
    3. Bruno Verhasselt, Evelien Naessens, Veronique Stove
      Pages 327-339
    4. Beatriz Albella, José Carlos Segovia, Guillermo Guenechea, Juan Antonio Bueren
      Pages 363-373
    5. Kohichiro Tsuji, Takahiro Ueda, Yasuhiro Ebihara
      Pages 387-395
  6. Maturation and Differentiation of Dendritic Cells

    1. Front Matter
      Pages 397-471
    2. Deanne M. R. Lathers, Nicholas Achille, M. Rita I. Young
      Pages 409-416
    3. Lina Matera, Alessandra Galetto
      Pages 417-426
    4. Pawel Kalinski, Pedro Vieira, Joost H. N. Schuitemaker, Quan Cai, Martien Kapsenberg
      Pages 427-436
    5. Mohamad Mohty, Béatrice Gaugler, Daniel Olive
      Pages 463-472
  7. Back Matter
    Pages 473-478

About this book


Enormous effort has been spent developing many new laboratory techniques for studying immune cells and the immune system, not only to increase fundamental understanding, but also to procure therapeutic success in a clinical setting. In Cytokines and Colony Stimulating Factors: Methods and Protocols, leading laboratory experts describe for the novice investigator a host of novel technologies and molecular techniques specifically designed to study cellular immunology and promote its gene therapy applications. Presented in step-by-step detail to ensure ready reproducibility, these protocols range from flow cytometric techniques to detect cytokines and growth factors in different specimens, to methods for generating and expanding dendritic cells and hematopoietic progenitors from different origins. Each proven method has been fine-tuned for experimental successs by the investigator who developed it, and includes notes on troubleshooting and avoiding potential pitfalls. Although the protocols require little previous experience, even highly skilled researchers will augment their experimental range with many novel timesaving techniques.
Cutting-edge and highly practical, Cytokines and Colony Stimulating Factors: Methods and Protocols provides researchers a carefully evaluated set of powerful tools for successful experimental research on cytokines and their role in gene therapy.

Editors and affiliations

  • Dieter Körholz
    • 1
  • Wieland Kiess
    • 1
  1. 1.Department of PediatricsUniversity of LeipzigLeipzigGermany

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2003
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-035-9
  • Online ISBN 978-1-59259-345-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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