© 2003

Protein Sequencing Protocols

  • Bryan John Smith

Part of the Methods in Molecular Biology™ book series (MIMB, volume 211)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Bryan John Smith, Paul Tempst
    Pages 1-18
  3. Bryan John Smith
    Pages 49-61
  4. Bryan John Smith
    Pages 63-82
  5. G. Brent Irvine, Ian Davidson
    Pages 123-131
  6. Alan J. Smith
    Pages 133-141
  7. Andrea Scaloni, Maurizio Simmaco, Francesco Bossa
    Pages 169-180
  8. Andrew J. Reason
    Pages 181-194
  9. Fiona M. Greer, Howard R. Morris
    Pages 195-210

About this book


Bryan John Smith updates his much-acclaimed first edition of Protein Sequencing Protocols with new and refreshed techniques for determining the sequence of proteins and peptides. This edition includes not only novel approaches to the validation of quality assurance methods, reflecting the current importance of biopharmaceuticals, but also offers a guide to the analysis of protein sequence information via the powerful new tools of bioinformatics. Each readily reproducible protocol contains detailed step-by-step instructions to ensure successful experimental results, along with notes on troubleshooting and how to avoid pitfalls. Valuable appendices summarize changes in mass arising from protein modification, as well as consensus sequences for protein modification.
Comprehensive and up-to-date, Protein Sequencing Protocols, Second Edition, provides both novice and expert investigators alike a ready source of easy-to-follow protocols that simplify choosing the most appropriate method for protein sequence determination and thus help assure robust results.

Editors and affiliations

  • Bryan John Smith
    • 1
  1. 1.Celltech ChiroscienceSloughUK

Bibliographic information

  • Book Title Protein Sequencing Protocols
  • Editors Bryan John Smith
  • Series Title Methods in Molecular Biology™
  • DOI
  • Copyright Information Humana Press 2003
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-0-89603-975-9
  • Softcover ISBN 978-1-61737-284-1
  • eBook ISBN 978-1-59259-342-2
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 2
  • Number of Pages XII, 493
  • Number of Illustrations 0 b/w illustrations, 0 illustrations in colour
  • Topics Biochemistry, general
  • Buy this book on publisher's site
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"...this book would certainly benefit readers who are new to the field as well as those more experienced as it serves as a convenient collection of tried and trusted methods...the book gives valuable background and insight to the various techniques associated with protein sequencing, which are often used by those providing services from within a core facility to the wider population of life science researchers." -Proteomics

"A positive feature of this book is that the fact that minute details, which are essential for successful implementation of an analytical method are provided. This book can serve as a useful reference for laboratories which mainly utilize chemical methods for protein analysis." -Pharmaceutical Research

From Reviews of the First Edition...

"...full of valuable information..." -Biochemical Education
"...provides very useful resources for those embarking on protein identification or characterization projects..." -FEBS Letters
" extremely useful compendium of protein analysis protocols..." -Nahrung-Food
"...well organized and accessible..." -Journal of the American Chemical Society

"The overall result is a concise and highly informative laboratory manual aimed at both operators and users of protein analysis facilities providing an understanding of what techniques involve, how samples should be prepared and in what form and amounts they should be submitted to achieve the most successful data. This publication has certainly proved to be a valuable asset to my laboratory and I would recommend it not only to those in protein sequencing and mass spectrometry laboratories, but also anyone with protein chemistry applications and interests."-Molecular Biotechnology