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Transgenic Mouse Methods and Protocols

  • Book
  • © 2002

Overview

Editors:
  1. Marten H. Hofker

    1. Department of Molecular Genetics, University of Maastricht, Maastricht, The Netherlands

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  2. Jan Deursen

    1. Department of Pediatrics and Adolescent Medicine, Mayo Clinic, Rochester, MN

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 209)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Introduction: The Use of Transgenic Mice in Biomedical Research

    • Marten H. Hofker
    Pages 1-8

  3. Genetic Modification of the Mouse

    • J. Willem Voncken
    Pages 9-34

  4. In Vitro Fertilization/Cryopreservation

    • Susan Marschall, Martin Hrabě de Angelis
    Pages 35-50

  5. Transgene Design

    • J. Willem Voncken
    Pages 51-67

  6. Generating Conditional Mouse Mutants via Tetracycline-Controlled Gene Expression

    • Kai Schönig, Hermann Bujard
    Pages 69-104

  7. Manipulating Large Insert Clones for Transgenesis

    • Sally P. A. McCormick, Catherine Y. Y. Liu, Stephen G. Young, Lars B. Nielsen
    Pages 105-123

  8. Reporter Molecules in Genetically Engineered Mice

    • Thomas L. Saunders
    Pages 125-143

  9. Gene Targeting in Mouse Embryonic Stem Cells

    • Jan van Deursen
    Pages 145-158

  10. Conditional Knockout Mice

    • Ralf Kühn, Frieder Schwenk
    Pages 159-185

  11. Knockin Approaches

    • Anton J. M. Roebroek, Xiaosheng Wu, Richard J. Bram
    Pages 187-200

  12. Aggregation of Embryos and Embryonic Stem Cells

    • Ronald Maatman, Marina Gertsenstein, Emile de Meijer, Andras Nagy, Kristina Vintersten
    Pages 201-230

  13. Andenovirus-Mediated Gene Transfer

    • Ko Willems van Dijk, Kyriakos E. Kypreos, Christine d’Oliveira, Frits J. Fallaux
    Pages 231-247

  14. Random ENU Mutagenesis

    • Dian Soewarto, Véronique Blanquet, Martin de Hrabě Angelis
    Pages 249-266

  15. Autopsy and Histologic Analysis of the Transgenic Mouse

    • Marion J. J. Gijbels, Menno P. J. de Winther
    Pages 267-279

  16. Bone Marrow Transplantations to Study Gene Function in Hematopoietic Cells

    • Menno P. J. de Winther, Peter Heeringa
    Pages 281-292

  17. Quantification of Atherosclerosis in Mice

    • Alan Daugherty, Stewart C. Whitman
    Pages 293-309

  18. Genetically Modified Mice in Cancer Research

    • David A. Largaespada
    Pages 311-332

  19. Neuropathobiology in Transgenic Mice

    • Jo Van Dorpe, Kurt Spittaels, Chris Van den Haute, Ilse Dewachter, Dieder Moechars, Hugo Geerts et al.
    Pages 333-361

  20. Back Matter

    Pages 363-374
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About this book

Marten Hofker and Jan van Deursen have assembled a multidisciplinary collection of readily reproducible methods for working with mice, and particularlyfor generating mouse models that will enable us to better understand gene function. Described in step-by-step detail by highly experienced investigators, these proven techniques include new methods for conditional, induced knockout, and transgenic mice, as well as for working with mice in such important research areas as immunology, cancer, and atherosclerosis. Such alternative strategies as random mutagenesis and viral gene transduction for studying gene function in the mouse are also presented.

Reviews

"...highly recommendable to cyto- and histochemist researchers, since they can easily find the way to enrich the instrumentarium of their own conceptual tools." - European Journal of Histochemistry

Editors and Affiliations

  • Department of Molecular Genetics, University of Maastricht, Maastricht, The Netherlands

    Marten H. Hofker

  • Department of Pediatrics and Adolescent Medicine, Mayo Clinic, Rochester, MN

    Jan Deursen

Bibliographic Information

  • Book Title: Transgenic Mouse Methods and Protocols

  • Editors: Marten H. Hofker, Jan Deursen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592593402

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2002

  • eBook ISBN: 978-1-59259-340-8Published: 04 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: 392

  • Number of Illustrations: 76 b/w illustrations, 4 illustrations in colour

  • Topics: Cell Biology

  • Industry Sectors: Biotechnology, Pharma

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