Transgenic Mouse

Methods and Protocols

  • Marten H. Hofker
  • Jan van Deursen

Part of the Methods in Molecular Biology™ book series (MIMB, volume 209)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. J. Willem Voncken
    Pages 9-34
  3. Susan Marschall, Martin Hrabě de Angelis
    Pages 35-50
  4. J. Willem Voncken
    Pages 51-67
  5. Sally P. A. McCormick, Catherine Y. Y. Liu, Stephen G. Young, Lars B. Nielsen
    Pages 105-123
  6. Thomas L. Saunders
    Pages 125-143
  7. Jan van Deursen
    Pages 145-158
  8. Ralf Kühn, Frieder Schwenk
    Pages 159-185
  9. Anton J. M. Roebroek, Xiaosheng Wu, Richard J. Bram
    Pages 187-200
  10. Ronald Maatman, Marina Gertsenstein, Emile de Meijer, Andras Nagy, Kristina Vintersten
    Pages 201-230
  11. Ko Willems van Dijk, Kyriakos E. Kypreos, Christine d’Oliveira, Frits J. Fallaux
    Pages 231-247
  12. Dian Soewarto, Véronique Blanquet, Martin de Hrabě Angelis
    Pages 249-266
  13. Marion J. J. Gijbels, Menno P. J. de Winther
    Pages 267-279
  14. Menno P. J. de Winther, Peter Heeringa
    Pages 281-292
  15. Alan Daugherty, Stewart C. Whitman
    Pages 293-309
  16. David A. Largaespada
    Pages 311-332
  17. Jo Van Dorpe, Kurt Spittaels, Chris Van den Haute, Ilse Dewachter, Dieder Moechars, Hugo Geerts et al.
    Pages 333-361
  18. Back Matter
    Pages 363-374

About this book


Genetically very similar to the human species, mice play an important role in biomedical research and have served as experimental models for a wide variety of pathologies, including cancer, cardiovascular diseases, and behavioral disorders. In Transgenic Mouse Methods and Protocols, Marten Hofker and Jan van Deursen have assembled a multidisciplinary collection of readily reproducible methods for working with mice, and particularly for generating mouse models that will enable us to better understand gene function. Described in step-by-step detail by highly experienced investigators, these proven techniques include new methods for conditional, induced knockout, and transgenic mice, as well as for working with mice in such important research areas as immunology, cancer, and atherosclerosis. Such alternative strategies as random mutagenesis and viral gene transduction for studying gene function in the mouse are also presented. Care is taken to make clear the details of the available approaches, as well as their limitations.
Up-to-date and highly practical, Transgenic Mouse Methods and Protocols demonstrates clearly for both novice and expert investigators how to make novel transgenic mouse models, and how to use them effectively to understand the role of gene function in human health and disease.

Editors and affiliations

  • Marten H. Hofker
    • 1
  • Jan van Deursen
    • 2
  1. 1.Department of Molecular GeneticsUniversity of MaastrichtMaastrichtThe Netherlands
  2. 2.Department of Pediatrics and Adolescent MedicineMayo ClinicRochesterMN

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2002
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-915-5
  • Online ISBN 978-1-59259-340-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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