© 2003

E. coli

Shiga Toxin Methods and Protocols

  • Dana Philpott
  • Frank Ebel

Part of the Methods in Molecular Medicine™ book series (MIMM, volume 73)

Table of contents

  1. Front Matter
    Pages i-xi
  2. James C. Paton, Adrienne W. Paton
    Pages 9-26
  3. Martin Bitzan, Helge Karch
    Pages 27-43
  4. Adrienne W. Paton, James C. Paton
    Pages 45-54
  5. Haruo Watanabe, Jun Terajima, Hidemasa Izumiya, Sunao Iyoda
    Pages 55-65
  6. Michael Bülte
    Pages 67-74
  7. Lothar H. Wieler, Rolf Bauerfeind
    Pages 75-89
  8. Frank Ebel, Dana Philpott
    Pages 91-98
  9. Tobias A. Oelschlaeger, Ulrich Dobrindt, Britta Janke, Barbara Middendorf, Helge Karch, Jörg Hacker
    Pages 99-112
  10. Soudabeh Djafari, Nadja D. Hauf, Judith F. Tyczka
    Pages 113-124
  11. Stuart Knutton
    Pages 137-149
  12. Herbert Schmidt, Roland Benz
    Pages 151-163
  13. Clifford A. Lingwood
    Pages 165-186
  14. Anita Nutikka, Beth Binnington-Boyd, Clifford A. Lingwood
    Pages 187-195
  15. Anita Nutikka, Beth Binnington-Boyd, Clifford A. Lingwood
    Pages 197-208
  16. Frédéric Mallard, Ludger Johannes
    Pages 209-220

About this book


Shiga toxin-producing Escherichia coli (STEC) and its isolated cytotoxin (Stx), also known as "hamburger E. coli" are continuing sources of significant-and sometime fatal-human and animal infections. In E. coli: Shiga Toxin Methods and Protocols, a multidisciplinary panel of leading experimentalists details the key protocols for the diagnosis and study of STEC and Stx pathogenesis. Described in step-by-step detail, these readily reproducible techniques range from those for the diagnosis and detection of STEC bacteria in patient and animal samples, to those for studying the cellular microbiology of STEC infections, especially host-pathogen interactions and the hemolysin of STEC. There are also protocols for studying the details of Shiga toxin (Stx) biology-from the purification of the toxin to the effects of Stx on various host cell functions-and for exploring STEC-mediated disease in various animal models. Each protocol has been thoroughly tested and optimized to produce robust and successful experimental results, and includes troubleshooting tips, advice on avoiding pitfalls, and alternate methods.
Comprehensive and up-to-date, E. coli: Shiga Toxin Methods and Protocols offers bacteriologists and medical microbiologists today's most powerful tools for illuminating the molecular and cellular microbiology of STEC's bacterial pathogenesis, as well as the mechanisms of its toxicity.

Editors and affiliations

  • Dana Philpott
    • 1
  • Frank Ebel
    • 2
  1. 1.Groupe d&'Immunité Innée et SignalisationInstitut PasteurParisFrance
  2. 2.Max-von-Pettenkofer-Institut, BakteriologieMunichGermany

Bibliographic information

  • Book Title E. coli
  • Book Subtitle Shiga Toxin Methods and Protocols
  • Editors Dana Philpott
    Frank Ebel
  • Series Title Methods in Molecular Medicine™
  • DOI
  • Copyright Information Humana Press 2003
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-0-89603-939-1
  • Softcover ISBN 978-1-61737-264-3
  • eBook ISBN 978-1-59259-316-3
  • Series ISSN 1543-1894
  • Series E-ISSN 1940-6037
  • Edition Number 1
  • Number of Pages XII, 352
  • Number of Illustrations 0 b/w illustrations, 0 illustrations in colour
  • Topics Microbiology
  • Buy this book on publisher's site
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"The authors should be thanked for providing this valuable resource tool that is highly recommended to microbiologists, molecular biologists and to all who are interested in bacterial toxin action. All medical libraries should house a copy of this text. The book also will serve as an excellent teaching guide for students and fellows particularly in postgraduate programs." -Folia Microbiologica