Molecular Cytogenetics

Protocols and Applications

  • Yao-Shan Fan

Part of the Methods in Molecular Biology™ book series (MIMB, volume 204)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Basic Concepts and Techniques

    1. Front Matter
      Pages 1-1
    2. Yao-Shan Fan
      Pages 3-20
    3. Larry E. Morrison, Ramesh Ramakrishnan, Teresa M. Ruffalo, Kim A. Wilber
      Pages 21-40
    4. Ramesh Ramakrishnan, Larry E. Morrison
      Pages 41-49
    5. Sue Van Stedum, Walter King
      Pages 51-63
  3. Evolving Techniques and Applications

    1. Front Matter
      Pages 65-65
    2. Xin-Yuan Guan
      Pages 67-76
    3. Jørn Koch
      Pages 77-84
    4. Jane Bayani, Jeremy A. Squire
      Pages 85-104
    5. Syed M. Jalal, Mark E. Law
      Pages 105-120
    6. Vicki L. Hopwood, Christi J. Hada, Armand B. Glassman
      Pages 133-142
    7. Jaennelle Kraan, Anne R. M. von Bergh, Karin Kleiverda, Jan-Willem Vaandrager, Ekaterina S. Jordanova, Anton K. Raap et al.
      Pages 143-153
    8. Samantha J. L. Knight, Jonathan Flint
      Pages 155-179
    9. Laurence Colleaux, Solange Heuertz, Florence Molinari, Marlene Rio
      Pages 181-189
    10. Ben Beheshti, Paul C. Park, Ilan Braude, Jeremy A. Squire
      Pages 191-207
  4. Special Applications in Chromosomal Disorders

    1. Front Matter
      Pages 209-209
    2. Michael D. Graf, Stuart Schwartz
      Pages 211-218
    3. Baruch Feldman, Ayala Aviram-Goldring, Mark I. Evans
      Pages 219-241
    4. Farideh Z. Bischoff, Dianne X. Dang, Dorothy E. Lewis, Joe Leigh Simpson
      Pages 243-258
    5. Yury Verlinsky, Jeanine Cieslak, Anver Kuliev
      Pages 259-273
    6. Stuart Schwartz, Michael D. Graf
      Pages 275-290
    7. Wendy P. Robinson
      Pages 291-298
    8. Héléne Bruyére, Evica Rajcan-Separovic, Dagmar K. Kalousek
      Pages 299-307
  5. Special Applications in Oncology

    1. Front Matter
      Pages 309-309
    2. Bae-Li Hsi, Sheng Xiao, Jonathan A. Fletcher
      Pages 343-351
    3. Rosemary E. Mueller, Frances P. O’Malley
      Pages 353-367
    4. Marjan M. Weiss, Mari A. J. A. Hermsen, Gerrit A. Meijer, Paul J. van Diest
      Pages 369-378
    5. Yanming Zhang, Brigitte Schlegelberger
      Pages 379-390
    6. Xiao-Ning Chen, Julie R. Korenberg
      Pages 391-403
  6. Back Matter
    Pages 405-411

About this book


With the proliferation of fluorescence in situ hybridization (FISH) techniques as a powerful diagnostic tool in many areas of medicine, the variety and complexity of tests pose bewildering choices even for long-experienced laboratory technologists and physicians. In Molecular Cytogenetics: Protocols and Applications, Yao-Shan Fan and a panel of senior scientists and pioneering researchers describe in step-by-step fashion not only the leading FISH techniques, but also those molecular technologies beyond FISH now available for diagnostic services in genetics and oncology. The methods include labeling FISH probes for DNA and RNA targets, fluorescence genotyping, CGH microarray, spectral karyotyping/multicolor FISH, and primed in situ labeling. There are also techniques for multicolor fiber FISH, multi-telomere FISH, prenatal diagnosis using maternal blood, and preimplantation diagnosis. Oncological methods include simultaneous fluorescence immunophenotyping and FISH for leukemia and lymphoma, HER2 amplification in breast cancer, and CAC/PAC for cancer cytogenetics. Each easy-to-follow protocol offers first hand advice on how to obtain the best results, to avoid pitfalls, and to troubleshoot problems. Many protocols discuss possible future applications of the technology and explain its sensitivity and limitations.
Cutting-edge and highly practical, Molecular Cytogenetics: Protocols and Applications offers laboratory physicians and scientists today's most comprehensive and up-to-date collection of FISH protocols for cancer diagnosis and investigation, plus an array of the emerging molecular technologies beyond FISH.

Editors and affiliations

  • Yao-Shan Fan
    • 1
    • 2
  1. 1.London Health Sciences CenterLondon
  2. 2.The University of Western OntarioOntarioCanada

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2003
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-006-9
  • Online ISBN 978-1-59259-300-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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