Peptide Nucleic Acids

Methods and Protocols

  • Peter E. Nielsen

Part of the Methods in Molecular Biology book series (MIMB, volume 208)

Table of contents

  1. Front Matter
    Pages i-x
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Peter E. Nielsen
      Pages 3-26
  3. Chemistry

    1. Front Matter
      Pages 27-27
    2. Frederik Beck
      Pages 29-41
    3. Satish Kumar Awasthi, Peter E. Nielsen
      Pages 43-52
    4. Satish Kumar Awasthi, Peter E. Nielsen
      Pages 53-57
    5. Tommi Ratilainen, Bengt Nordén
      Pages 59-88
  4. PNA Technology

    1. Front Matter
      Pages 89-89
    2. Thomas Bentin, Georg I. Hansen, Peter E. Nielsen
      Pages 91-107
    3. Thomas Bentin, Peter E. Nielsen
      Pages 111-118
    4. Vadim V. Demidov, Maxim D. Frank-Kamenetskii
      Pages 119-130
    5. Miaomiao Wang, Bruce A. Armitage
      Pages 131-142
  5. Diagnostics

    1. Front Matter
      Pages 143-143
    2. Deborah G. Murdock, Douglas C. Wallace
      Pages 145-164
    3. Marcus Schuermann, Moira Behn
      Pages 165-179
    4. Brett Williams, Henrik Stender, James M. Coull
      Pages 181-193
  6. Antisense and Antigene

    1. Front Matter
      Pages 209-209
    2. Dwaine A. Braasch, David R. Corey
      Pages 211-223
    3. Margus Pooga, Ursel Soomets, Tamas Bartfai, Ülo Langel
      Pages 225-236
    4. Niels Erik MΦllegaard, Peter E. Nielsen
      Pages 249-256
  7. Animal Studies

  8. Back Matter
    Pages 271-274

About this book


Peptide nucleic acid (PNA) technology continues to grow in importance throughout molecular biology, genetic diagnostics, and molecular medicine. In Peptide Nucleic Acids: Methods and Protocols, Peter Eigil Nielsen has assembled a critically evaluated collection of PNA protocols that are either already well established around the world, such as PCR-clamping and in situ hybridization, or that display promise of significant future impact. Basic methods for PNA oligomer synthesis and analysis have also been included. Written by experts with hands-on experience in the methods they describe, these readily reproducible protocols contain detailed step-by-step instructions, tips on avoiding pitfalls and on extending the method to other situations, and introductory material explaining the theory behind the process.
Up-to-date and highly practical, Peptide Nucleic Acids: Methods and Protocols provides for both novice and experienced researchers a thorough and easy introduction to the optimal use of today's PNA technology across a wide variety of fields, including molecular biology, medicinal chemistry, genetic diagnostics, and drug development.

Editors and affiliations

  • Peter E. Nielsen
    • 1
  1. 1.Center for Biomolecular Recognition The Panum InstituteUniversity of CopengagenCopenhagenDenmark

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2002
  • Publisher Name Springer, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-976-6
  • Online ISBN 978-1-59259-290-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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