Mitochondrial DNA

Methods and Protocols

  • William C. Copeland

Part of the Methods in Molecular Biology™ book series (MIMB, volume 197)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Methods for the Analysis of MTDNA

    1. Front Matter
      Pages 1-1
    2. Douglas C. Wallace
      Pages 3-54
    3. Yuichi Michikawa, Giuseppe Attardi
      Pages 75-92
    4. Shuji Nomoto, Montserrant Sanchez-Cespedes, David Sidransky
      Pages 107-118
    5. Steven J. Zullo
      Pages 119-128
    6. Françoise Foury
      Pages 139-149
    7. Micheline K. Strand, William C. Copeland
      Pages 151-157
    8. Janine H. Santos, Bhaskar S. Mandavilli, Bennett Van Houten
      Pages 159-176
    9. Mary K. Thorsness, Karen H. White, Peter E. Thorsness
      Pages 177-186
    10. Heather E. Lorimer
      Pages 187-196
  3. Methods for the Analysis of proteins involved in MTDNA Transactions

    1. Front Matter
      Pages 197-197
    2. Samuel E. Bennett, Mary Jane Shroyer, Jung-Suk Sung, Dale W. Mosbaugh
      Pages 211-225
    3. Simon G. Nyaga, Vilhelm A. Bohr
      Pages 227-244
    4. Robert K. Naviaux
      Pages 259-271
    5. Silja Kuusk, Tiina Sedman, Juhan Sedman
      Pages 303-316
    6. Robert L. Low, Mariana Gerschenson
      Pages 331-349
    7. Allison W. Dobson, Mark R. Kelley, Glenn L. Wilson, Susan P. LeDoux
      Pages 351-362
    8. Steven C. Ghivizzani, Cort S. Madsen, Chandramohan V. Ammini, William W. Hauswirth
      Pages 363-375
    9. Brett A. Kaufman, Scott M. Newman, Philip S. Perlman, Ronald A. Butow
      Pages 377-389
    10. Mats Ekstrand, Nils-Göran Larsson
      Pages 391-400
  4. Back Matter
    Pages 401-414

About this book


Mutations within mitochondrial DNA (mtDNA) and the nuclear genes involved in the maintenance of mitochondrial DNA have been linked to a wide range of human diseases, including several of the most common diseases of aging. In Mitochondrial DNA: Methods and Protocols internationally recognized authorities describe in great detail the methods they have perfected to analyze mtDNA and the proteins involved in its maintenance. The analytical techniques cover the purification of mtDNA from a variety of sources and the analysis of DNA for both deletions, point mutations, and damage, for replication intermediates, and for following the fate of mtDNA outside of the mitochondria. Additional analytical methods are presented for analyzing the proteins and enzymes that maintain mtDNA. Each readily reproducible protocol includes step-by-step instructions, tips on avoiding pitfalls and extending the method to other situation, and introductory material explaining the theory behind the process.
Comprehensive and timely, Mitochondrial DNA: Methods and Protocols offers both basic and clinical researchers proven cutting-edge methods for analyzing the role mtDNA plays in the aging process, apoptosis, and possibly some cancers, and for investigating the cause of mitochondrial dysfunction and disease.

Editors and affiliations

  • William C. Copeland
    • 1
  1. 1.Laboratory of Molecular GeneticsNational Institute of Environmental Health Sciences, National Institutes of Health

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2002
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-972-8
  • Online ISBN 978-1-59259-284-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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