GTPase Protocols

The Ras Superfamily

  • Ed Manser
  • Thomas Leung

Part of the Methods in Molecular Biology™ book series (MIMB, volume 189)

Table of contents

  1. Front Matter
    Pages i-xi
  2. General Protocols

    1. Front Matter
      Pages 1-1
    2. Susan J. M. Smith, Katrin Rittinger
      Pages 13-24
    3. Mohammad Reza Ahmadian, Alfred Wittinghofer, Christian Herrmann
      Pages 45-63
  3. Specific Methods

    1. Front Matter
      Pages 65-65
    2. Herbert Archer, Dafna Bar-Sagi
      Pages 67-73
    3. Hiroshi Maruta, Hong He, Thao Nheu
      Pages 75-86
    4. Angeliki Malliri, Jean P. Ten Klooster, Cristina Olivo, John G. Collard
      Pages 99-109
    5. Masaki Fukata, Masato Nakagawa, Shinya Kuroda, Kozo Kaibuchi
      Pages 121-128
    6. Jackie Banyard, Marc Symons
      Pages 129-140
    7. Hiroyuki Nakanishi, Takuya Sasaki, Yoshimi Takai
      Pages 143-156
    8. Paul A. Randazzo, Henry M. Fales
      Pages 169-179
    9. Gustavo Pacheco-Rodriguez, Joel Moss, Martha Vaughan
      Pages 181-189
    10. Julie G. Donaldson
      Pages 191-198
    11. Irit Huber, Edna Cukierman, Miriam Rotman, Dan Cassel
      Pages 199-206
    12. Amy M. Brownawell, James M. Holaska, Ian G. Macara, Bryce M. Paschal
      Pages 209-229
    13. Sofia B. Lizarraga, Yixian Zheng, Andrew R. Wilde
      Pages 247-260
  4. Back Matter
    Pages 331-345

About this book


Small GTPase binding proteins (GTPases) are an ancient group of proteins that play key roles in almost every aspect of cell biology, from cell proliferation to nuclear transport. In GTPase Protocols: The Ras Superfamily, Edward J. Manser and Thomas Leung have collected the key techniques currently in use to probe the function of these ubiquitous proteins both in vitro and in vivo. Presented in a format that ensures ready reproducibility by accomplished experimentalists who have refined the various methods in their laboratories, each technique includes step-by-step instructions, tips on avoiding pitfalls and troubleshooting, and ancillary notes explaining how to adapt each procedure in the event of problems. The methods cover the spectrum of core techniques required for the five major GTPase subfamilies (Ras, Rho, Rab, Arf, and Ran) and permit a diversity of applications ranging from structural studies on a GTPase to real time in vivo analysis.
Timely and highly practical, GTPase Protocols: The Ras Superfamily illuminates the powerful techniques used by investigators today to study this special family of proteins that plays such important roles in human health and disease.

Editors and affiliations

  • Ed Manser
    • 1
  • Thomas Leung
    • 1
  1. 1.Glaxo-IMCB GroupInstitute of Molecular and Cell BiologySingapore

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2002
  • Publisher Name Springer, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-934-6
  • Online ISBN 978-1-59259-281-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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