Oxidants and Antioxidants

Ultrastructure and Molecular Biology Protocols

  • Donald Armstrong

Part of the Methods in Molecular Biology™ book series (MIMB, volume 196)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Techniques for Histological Identification

    1. Front Matter
      Pages 1-1
    2. E. Ann Ellis, Maria B. Grant
      Pages 3-12
    3. Kiyoshi Akeo, Tadahisa Hiramitsu, Keiichi Watanabe
      Pages 19-29
    4. Hideyuki J. Majima, Takako Nakanishi-Ueda, Toshihiko Ozawa
      Pages 31-34
    5. Jürgen Frank, Alfonso Pompella, Hans K. Biesalski
      Pages 35-40
    6. Alfonso Pompella, Silvia Dominici, Jürgen Frank, Hans K. Biesalski
      Pages 41-46
  3. Techniques for Subcellular Localization

  4. Techniques for Molecular Biology

    1. Front Matter
      Pages 97-97
    2. Ahmad Aljada, Husam Ghanim, Paresh Dandona
      Pages 99-103
    3. Ahmad Aljada, Husam Ghanim, Paresh Dandona
      Pages 105-110
    4. Gieri Camenisch, Roland H. Wenger, Max Gassmann
      Pages 117-129

About this book


It is now feasible to identify subcellular sites where oxidative stress reactions occur and to determine gene expression relevant to those complex pathways. In Oxidants and Antioxidants: Ultrastructure and Molecular Biology Protocols, a panel of world authorities present cutting-edge molecular and ultrastructure techniques for investigating the pathophysiology of oxidative stress. Described in step-by-step detail, these readily reproducible methods use the latest in immunodetection, X-ray diffraction, confocal microscopy, in situ hybridization, chemiluminescence, and cytofluorescence. Additional techniques take advantage of electron paramagnetic resonance, advanced glycation endproducts, Western blotting analysis, transcription factors, p47phox translocation, and microsphere delivery systems. The collection emphasizes the visual observation of free radicals or products altered by oxidative stress, and includes numerous in vivo, ex vivo, and in vitro applications. Two companion volumes, Oxidative Stress Biomarkers and Antioxidant Protocols (2002), and Free Radical and Antioxidant Protocols (1998), focus on the related biochemical and cellular biological methods.
Authoritative and highly practical, Oxidants and Antioxidants: Ultrastructure and Molecular Biology Protocols provides a comprehensive collection of powerful ultrastructure and molecular tools for the evaluation of metabolic disorders linked to oxidative stress and the aging process.

Editors and affiliations

  • Donald Armstrong
    • 1
  1. 1.University of FloridaGainesville

Bibliographic information

  • DOI https://doi.org/10.1385/1592592740
  • Copyright Information Humana Press 2002
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-851-6
  • Online ISBN 978-1-59259-274-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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