© 1999

Flavoprotein Protocols

  • Stephen K. Chapman
  • Graeme A. Reid

Part of the Methods in Molecular Biology book series (MIMB, volume 131)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Alessandro Aliverti, Bruno Curti, Maria A. Vanoni
    Pages 9-23
  3. Andrew W. Munro, Michael A. Noble
    Pages 25-48
  4. Willem J. H. van Berkel, Jacques A. E. Benen, Michel H. M. Eppink, Marco W. Fraaije
    Pages 61-85
  5. Andrew W. Munro, Sharon M. Kelly, Nicholas C. Price
    Pages 111-123
  6. Iain D. G. Macdonald
    Pages 125-138
  7. Jacques Vervoort, Marco Hefti
    Pages 139-147
  8. Florence Lederer, Heinz Rüterjans, Garrit Fleischmann
    Pages 149-155
  9. Dale Edmondson, Sandro Ghisla
    Pages 157-179
  10. R. E. Sharp, P. L. Dutton
    Pages 195-206
  11. Lars Ridder, Hans Zuilhof, Jacques Vervoort, Ivonne M. C. M. Rietjens
    Pages 207-228
  12. Katja Becker, Markus Schirmer, Stefan Kanzok, R. H. Schirmer
    Pages 229-245
  13. Back Matter
    Pages 247-256

About this book


As a scientist with an interest in proteins you will, at some time in your career, isolate an enzyme that turns out to be yellow—or perhaps you already have. Alternatively, you may identify a polypeptide sequence that is related to known flavin-containing proteins. This may, or may not, be your first encounter with flavoproteins. However, even if you are an old hand in the field, you may not have exploited the full range of experimental approaches applicable to the study of flavoproteins. We hope that Flavoprotein Protocols will encourage you to do so. In this volume we have sought to bring together a range of experimental methods of value to researchers with an interest in flavoproteins, whether or not these researchers have experience in this area. A broad range of techniques, from the everyday to the more specialized, is described by scientists who are experts in their fields and who have ext- sive practical experience with flavoproteins. The wide range of approaches, from wet chemistry to dry computation, has, as a consequence, demanded a range of formats. Where appropriate (particularly for analytical methods) the protocol described is laid out in easy-to-follow steps. In other cases (e. g. , the more advanced spectroscopies and computational methods) it is far more apt to describe the general approach and relevance of the methods. We hope this wide-ranging approach will sow the seeds of many future collaborations - tween laboratories and further our knowledge and understanding of how f- voproteins work.

Editors and affiliations

  • Stephen K. Chapman
    • 1
  • Graeme A. Reid
    • 1
  1. 1.University of EdinburghEdinburghUK

Bibliographic information

  • Book Title Flavoprotein Protocols
  • Editors Steven K. Chapman
    Graeme A. Reid
  • Series Title Methods in Molecular Biology
  • DOI
  • Copyright Information Humana Press 1999
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Softcover ISBN 978-1-61737-158-5
  • eBook ISBN 978-1-59259-266-1
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XII, 256
  • Number of Illustrations 0 b/w illustrations, 0 illustrations in colour
  • Topics Biochemistry, general
  • Buy this book on publisher's site
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"A detailed analysis of flavoproteins will be of value for investigators working with flavin enzymes whether studying mechanisms or searching for inhibitors. Chapters on chemical syntheses and the use of analogs provide useful information on approaches to both of these areas.."-Doodys Health Sciences Book Review Journal