Protein Lipidation Protocols

  • Michael H. Gelb

Part of the Methods in Molecular Biology book series (MIMB, volume 116)

Table of contents

  1. Front Matter
    Pages i-x
  2. Jun Liu, Jan E. Schnitzer
    Pages 61-72
  3. Fulvio Reggiori, Elisabeth Canivenc-Gansel, Andreas Conzelmann
    Pages 91-105
  4. Douglas A. Andres, Dean C. Crick, Brian S. Finlin, Charles J. Waechter
    Pages 107-123
  5. Alberto Corsini, Christopher C. Farnsworth, Paul McGeady, Michael H. Gelb, John A. Glomset
    Pages 125-144
  6. Rajala V. S. Raju, Rajendra K. Sharma
    Pages 193-211
  7. Back Matter
    Pages 229-240

About this book


In Protein Lipidation Protocols, Michael Gelb brings together a collection of readily reproducible techniques for studying protein lipidation, the covalent attachment of lipids to proteins. These cutting-edge methods-many never published before in a "hands-on" format-deal with glycosyl phosphatidylinositol (GPI)-containing compounds, protein fatty acylation, and protein prenylation. Included are novel techniques for determining the chemical structure of GPI-anchors, for radiolabeling the prenyl groups of protein in eukaryotic cells, a tool for developing inhibitors of the protein farnesyltransferase, and for an exciting lysosomal enzyme that cleaves fatty acyl groups from proteins, the first fatty acylase discovered.
Protein Lipidation Protocols offers biochemists, cell and molecular biologists, medicinal chemists, and pharmaceutical researchers state-of-the-art tools for understanding the complex biochemistry of protein lipidation, as well as catalyzing the development of many important new biopharmaceuticals, including anticancer drugs.

Editors and affiliations

  • Michael H. Gelb
    • 1
  1. 1.University of WashingtonSeattle

Bibliographic information

  • DOI
  • Copyright Information Humana Press 1999
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-534-8
  • Online ISBN 978-1-59259-264-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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