Calcium Signaling Protocols

  • David G. Lambert

Part of the Methods in Molecular Biology™ book series (MIMB, volume 114)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. General

    1. Front Matter
      Pages 1-1
    2. Alec W. M. Simpson
      Pages 3-30
    3. Robert A. Hirst, Charlotte Harrison, Kazuyoshi Hirota, David G. Lambert
      Pages 31-39
  3. Specialist Measurement Systems

    1. Front Matter
      Pages 49-49
    2. Laura C. Mongan, Joanne Gormally, Andrew R. D. Hubbard, Christopher d’Lacey, Colin D. Ockleford
      Pages 51-74
    3. Carmen Perez-Terzic, Marisa Jaconi, Lisa Stehno-Bittel
      Pages 75-91
    4. Elaine Sullivan, Emily M. Tucker, Ian L. Dale
      Pages 125-133
  4. Nonelectrophysiological measurement of Ca2+ channel activity

    1. Front Matter
      Pages 135-135
    2. Mercedes Villarroya, Manuela G. López, María F. Cano-Abad, Antonio G. García
      Pages 137-147
    3. Kazuyoshi Hirota, David G. Lambert
      Pages 149-161
  5. Measurement of Ins(1,4,5)P3 and Ca2+ Release from Intracellular Stores

  6. Specialist Measurement Techniques

    1. Front Matter
      Pages 267-267
    2. Leong L. Ng, Paulene A. Quinn
      Pages 279-286
    3. Raul Martinez-Zaguilan, Linda S. Tompkins, Robert J. Gillies, Ronald M. Lynch
      Pages 287-306
    4. Martin R. McAinsh, Irina Staxén
      Pages 307-321
  7. Ca2+ Sensitive Targets

    1. Front Matter
      Pages 323-323
    2. Rakesh Kakkar, Rajendra K. Sharma
      Pages 325-341
  8. Back Matter
    Pages 355-359

About this book


In Calcium Signaling Protocols, David Lambert and a panel of leading authorities present a wide range of experimental protocols for studying Ca2+ signaling. These optimized techniques cover the more common applications, including 45Ca2+ flux measurements, and basic fluorometric technology, as well as more sophisticated methods, including confocal microscopy and subcellular Ca2+ imaging. There are also methods-largely based on fluorescence measurement-to determine Ca2+ channel activity and the release of Ca2+ from intracellular stores. In addition, there are methods to assess Ca2+ -sensitive target site activity.
Calcium Signaling Protocols offers today's researchers readily reproducible laboratory methods that make it possible to examine the calcium signaling process in detail in a range of cells of animal and plant origin. These cutting-edge techniques will be of enormous value to all those working to understand not only cell signaling, but also the mode of action of a range of pharmacological agents.

Editors and affiliations

  • David G. Lambert
    • 1
  1. 1.University of LeicesterUK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 1999
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-597-3
  • Online ISBN 978-1-59259-250-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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