© 1999

Hemostasis and Thrombosis Protocols

  • David J. Perry
  • K. J. Pasi

Part of the Methods in Molecular Medicine™ book series (MIMM, volume 31)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. K. John Pasi
      Pages 3-21
  3. Basic Techniques

    1. Front Matter
      Pages 23-23
    2. David J. Perry
      Pages 25-30
    3. David J. Perry
      Pages 31-38
    4. David J. Perry
      Pages 39-48
    5. Helen L. Devereux
      Pages 55-61
    6. David J. Perry, Flora Peyvandi
      Pages 63-64
    7. Peter R. Winship, Jonathan R. K. Spray
      Pages 65-81
  4. Methods of Mutational Analysis

  5. Methods for Analyzing Inherited/Acquired Disorders of Hemostasis

    1. Front Matter
      Pages 131-131
    2. Naushin H. Waseem, Richard Bagnall, Peter M. Green, Francesco Giannelli
      Pages 133-149

About this book


In Hemostasis and Thrombosis Protocols, leading experimentalists describe in detail their proven, cutting-edge methods for research on hemostasis and thrombosis, as well as their diagnostics. The techniques range from widely used basic assays to methods that are specialized for mutational analysis and specific disorders. Readily reproducible, these powerful methods can be used to screen for such inherited disorders as hemophilia A and B, von Willebrands Disease, Factor XI and antithrombin deficiency, protein S deficiency, factor V Leiden mutation, and other problems. The protocols reflect the many major advances that have occurred in our understanding of the molecular genetics of normal hemostasis and it variants, as well as of the coagulation cascade itself.

Suitable for both diagnostic and research laboratories, Hemostasis and Thrombosis Protocols enables experienced and novice investigators alike readily to master the complexities of research on molecular hemostasis and thrombosis, as well as work toward the development of productive new molecular diagnostics.

Editors and affiliations

  • David J. Perry
    • 1
  • K. J. Pasi
    • 1
  1. 1.Royal Free HospitalLondonUK

Bibliographic information

  • Book Title Hemostasis and Thrombosis Protocols
  • Editors David J. Perry
    K. John Pasi
  • Series Title Methods in Molecular Medicine™
  • DOI
  • Copyright Information Humana Press 1999
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-0-89603-419-8
  • Softcover ISBN 978-1-4899-4313-2
  • eBook ISBN 978-1-59259-248-7
  • Series ISSN 1543-1894
  • Series E-ISSN 1940-6037
  • Edition Number 1
  • Number of Pages XIII, 368
  • Number of Illustrations 0 b/w illustrations, 0 illustrations in colour
  • Topics Pathology
  • Buy this book on publisher's site
Industry Sectors
Health & Hospitals


"...The book by Perry and Pasi confirms ...that with good recipes and kits the DNA analysis of disorders of thrombosis and hemostasis is indeed feasible for many diagnostic laboratories. The book describes in great detail a variety of laboratory protocols aimed primarily at analyzing inherited disorders of the hemostatic system. These protocols vary from simple procedures to detect factor V Leiden and prothrombin 20210 to elaborate techniques for finding mutations in disorders like hemophilia A or protein S deficiency. In addition ...this book is a useful book for those attempting to introduce or maintain DNA analysis in their laboratory...laboratory technicians may find many useful suggestions to improve their work."-Haemostasis

"Each cheater is presented in a recipe format so that the methods can e reproduced. All chapters subdivided into sections for easy reading: introduction, material, methods, and notes. ...The chapters on mutation detection in clotting factor deficiencies will be useful to hemostasis laboratories that are interested in carrier detection, prenatal diagnosis, or research. The chapters on von Willbrand factor multimer analysis, factor V Leiden mutation, an prothrombin G20210A mutation will be useful to hemostasis laboratories that want to set up these important diagnostic tests...This manual does well at meeting its goal of providing a broad overview of techniques used in the analysis of hemostatic disorders and providing strategies for future problem solving. It will be a great utility to hemostasis laboratories that wish to advance their research or diagnostic capabilities."-Modern Pathology

"very valuable for the laboratory staff in charge of gene analysis of known genetic defects in known diseases of hemostasis and thrombosis." - Bloodline