© 2000

Cytomegalovirus Protocols

  • John Sinclair

Part of the Methods in Molecular Medicine™ book series (MIMM, volume 33)

Table of contents

  1. Front Matter
    Pages i-x
  2. Andrew D. Yurochko, Eng-Shang Huang
    Pages 1-19
  3. Walter Hinderer, Bodo Plachter, Rolf Vornhagen
    Pages 21-37
  4. Anamaris M. Colberg-Poley, Vera E. Soltero, Richard-Fabian Schumacher
    Pages 67-77
  5. Stefan Flöss, Thomas Stamminger
    Pages 95-114
  6. Mark R. Wills, J. G. Patrick Sissons
    Pages 115-127
  7. Johan Neyts, Graciela Andrei, Robert Snoeck, Astrid Meerbach, Erik De Clercq
    Pages 129-152
  8. Richard F. Greaves
    Pages 153-171
  9. Back Matter
    Pages 173-182

About this book


In Cytomegalovirus Protocols (CMV), John Sinclair and a panel of expert investigators present a comprehensive collection of cellular and molecular techniques for the analysis of cytomegalovirus biology and its pathogenetic mechanisms. The methods-all described in step-by-step detail with ready reproducibility in mind-range from basic virus culture to complex molecular analysis of CMV structure and function. Included are methods for CMV detection using both immunological and biological techniques, methods for analyzing fundamental aspects of the CMV infection cycle, and methods for analyzing T cell response to cytomegalovirus infection in the human host.
Comprehensive and state-of-the-art, Cytomegalovirus Protocols provides investigators with a powerful collection of the key methods that are illuminating not only the basic biology of this complex and intriguing human herpesvirus, but also its significant role in the pathogenesis of human infectious diseases as well as their emergent therapies.

Editors and affiliations

  • John Sinclair
    • 1
  1. 1.University of Cambridge School of Clinical MedicineCambridgeUK

Bibliographic information

  • Book Title Cytomegalovirus Protocols
  • Editors John Sinclair
  • Series Title Methods in Molecular Medicine™
  • DOI
  • Copyright Information Humana Press 2000
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-0-89603-749-6
  • Softcover ISBN 978-1-61737-167-7
  • eBook ISBN 978-1-59259-244-9
  • Series ISSN 1543-1894
  • Series E-ISSN 1940-6037
  • Edition Number 1
  • Number of Pages X, 182
  • Number of Illustrations 0 b/w illustrations, 0 illustrations in colour
  • Topics Infectious Diseases
  • Buy this book on publisher's site
Industry Sectors
Health & Hospitals
Public Health
Internal Medicine & Dermatology


". . . detailed protocols with essential background material and seminal references for methods are discussed, ranging from immunologic and biochemical assays to techniques such as transfection and recombinant viral constructs. . . The book is clearly written; the best parts are the background introductions and the notes sections. . .this is a worthwhile addition to the library or personal collection of scientists, whenever HCMV or other herpes virus bench research is performed."-Doody's Health Science Book Review Journal

"I suspect this research protocols book will become e common place in cytomegalovirus labs across the globe and should serve to reinforce "in-house" methods texts...a useful laboratory manual for those coming into the field, as well as for experienced cytomegalovirologists wishing to broaden their technical expertise."-Microbiology Today

"This volume provides detailed laboratory protocols for a range of techniques relevant to human cytomegalovirus research...the volume is a high quality one, and I recommend it to those working in the field." - The Journal of Medical Microbiology