© 2002

Heme, Chlorophyll, and Bilins

Methods and Protocols

  • Alison G. Smith
  • Michael Witty

Part of the Springer Protocols Handbooks book series (SPH)

Table of contents

  1. Front Matter
    Pages i-ix
  2. Alison G. Smith, Michael Witty
    Pages 1-12
  3. Kevin M. Smith
    Pages 13-38
  4. Jerry C. Bommer, Peter Hambright
    Pages 39-67
  5. Martin J. Warren, Peter M. Shoolingin-Jordan
    Pages 69-93
  6. Angela Wilks
    Pages 157-184
  7. Harald Paulsen, Volkmar H. R. Schmid
    Pages 235-253
  8. Matthew J. Terry
    Pages 273-291
  9. Michael T. McDowell, J. Clark Lagarias
    Pages 293-309
  10. Back Matter
    Pages 335-340

About this book


Although researchers can profitably investigate heme, chlorophyll, and related tetrapyrroles in a wide range of academic and medical research programs, the handling and manipulation of these delicate compounds requires considerable skill and cross-boundary knowledge. In Heme, Chlorophyll, and Bilins: Methods and Protocols, an interdisciplinary panel of hands-on investigators overcomes these limitations by describing in detail how to work successfully with chlorophyll, heme, and bilins in biological, medical, chemical, and biochemical research. Each method is presented by a researcher who actually uses it on a daily basis and includes step-by-step instructions and pertinent tricks-of-the-trade that often make the difference between laboratory success and failure. Topics range from methods for the analysis of tetrapyrroles, heme, and hemoproteins, to the biosynthesis and the analysis of chlorophyll and bilins.
Timely and highly practical, Heme, Chlorophyll, and Bilins: Methods and Protocols is a gold-standard collection of readily reproducible techniques suitable for a wide range of researchers, whether it be a clinician studying photodynamic therapy, an ecologist studying the chlorophyll composition of leaves in a tropical forest, or a cell biologist investigating the function of specific hemoproteins.


biological biosynthesis crystal crystallization electron electron microscopy intermediate laboratory microscopy phase protein proteins purification reactions synthesis

Editors and affiliations

  • Alison G. Smith
    • 1
  • Michael Witty
    • 2
  1. 1.Department of Plant SciencesUniversity of CambridgeCambridgeUK
  2. 2.Department of BiochemistryUniversity of CambridgeCambridgeUK

Bibliographic information

  • Book Title Heme, Chlorophyll, and Bilins
  • Book Subtitle Methods and Protocols
  • Editors Alison Smith
    Michael Witty
  • Series Title Springer Protocols Handbooks
  • Series Abbreviated Title Springer Protocols Handbooks
  • DOI
  • Copyright Information Humana Press 2002
  • Publisher Name Humana Press
  • eBook Packages Springer Book Archive
  • Hardcover ISBN 978-1-58829-111-0
  • Softcover ISBN 978-1-61737-365-7
  • eBook ISBN 978-1-59259-243-2
  • Series ISSN 1949-2448
  • Series E-ISSN 1949-2456
  • Edition Number 1
  • Number of Pages IX, 340
  • Number of Illustrations 0 b/w illustrations, 0 illustrations in colour
  • Topics Plant Sciences
  • Buy this book on publisher's site
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"This handy reference book features a collection of techniques written by experts in the field for working successfully with the title classes of compounds." - Journal of the American Chemical Society