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Heme, Chlorophyll, and Bilins

Methods and Protocols

  • Book
  • © 2002

Overview

Editors:
  1. Alison G. Smith

    1. Department of Plant Sciences, University of Cambridge, Cambridge, UK

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    You can also search for this editor in PubMed   Google Scholar

  2. Michael Witty

    1. Department of Biochemistry, University of Cambridge, Cambridge, UK

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    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Springer Protocols Handbooks (SPH)

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Table of contents (14 protocols)

  1. Front Matter

    Pages i-ix
    Download chapter PDF

  2. Laboratory Methods for the Study of Tetrapyrroles

    • Alison G. Smith, Michael Witty
    Pages 1-12

  3. Syntheses of Tetrapyrroles

    • Kevin M. Smith
    Pages 13-38

  4. General Laboratory Methods for Tetrapyrroles

    • Jerry C. Bommer, Peter Hambright
    Pages 39-67

  5. Enzymatic Preparation of Tetrapyrrole Intermediates

    • Martin J. Warren, Peter M. Shoolingin-Jordan
    Pages 69-93

  6. Analysis of Biosynthetic Intermediates, 5-Aminolevulinic Acid to Heme

    • Chang Kee Lim
    Pages 95-109

  7. Analysis of Intermediates and End Products of the Chlorophyll Biosynthetic Pathway

    • Constantin A. Rebeiz
    Pages 111-155

  8. Analysis of Heme and Hemoproteins

    • Angela Wilks
    Pages 157-184

  9. Hemoproteins Purification and Characterization by Using Aqueous Two-Phase Systems

    • Daniel Forciniti
    Pages 185-208

  10. Structural Study of Heme Proteins by Electron Microscopy of 2-Dimensional Crystals

    • Terrence G. Frey
    Pages 209-234

  11. Analysis and Reconstitution of Chlorophyll-Proteins

    • Harald Paulsen, Volkmar H. R. Schmid
    Pages 235-253

  12. Two-Dimensional Crystallization of Chlorophyll Proteins

    • Georgios Tsiotis
    Pages 255-271

  13. Biosynthesis and Analysis of Bilins

    • Matthew J. Terry
    Pages 273-291

  14. Analysis and Reconstitution of Phytochromes

    • Michael T. McDowell, J. Clark Lagarias
    Pages 293-309

  15. Analysis and Reconstitution of Phycobiliproteins: Methods for the Characterization of Bilin Attachment Reactions

    • Wendy M. Schluchter, Donald A. Bryant
    Pages 311-334

  16. Back Matter

    Pages 335-340
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Keywords

About this book

In Heme, Chlorophyll, and Bilins: Methods and Protocols, an interdisciplinary panel of hands-on investigators describe in detail how to work successfully with chlorophyll, heme, and bilins in biological, medical, chemical, and biochemical research. Each method is presented by a researcher who actually uses it on a daily basis and includes step-by-step instructions and pertinent tricks-of-the-trade that often make the difference between laboratory success and failure. Topics range from methods for the analysis of tetrapyrroles,heme, and hemoproteins, to the biosynthesis and analysis of chlorophyll and bilins.

Reviews

"This handy reference book features a collection of techniques written by experts in the field for working successfully with the title classes of compounds." - Journal of the American Chemical Society

Editors and Affiliations

  • Department of Plant Sciences, University of Cambridge, Cambridge, UK

    Alison G. Smith

  • Department of Biochemistry, University of Cambridge, Cambridge, UK

    Michael Witty

Bibliographic Information

  • Book Title: Heme, Chlorophyll, and Bilins

  • Book Subtitle: Methods and Protocols

  • Editors: Alison G. Smith, Michael Witty

  • Series Title: Springer Protocols Handbooks

  • DOI: https://doi.org/10.1385/1592592430

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer Science+Business Media LLC 2002

  • Hardcover ISBN: 978-1-58829-111-0Published: 01 November 2001

  • Softcover ISBN: 978-1-61737-365-7Published: 05 November 2010

  • eBook ISBN: 978-1-59259-243-2Published: 07 June 2008

  • Series ISSN: 1949-2448

  • Series E-ISSN: 1949-2456

  • Edition Number: 1

  • Number of Pages: IX, 340

  • Topics: Plant Sciences

  • Industry Sectors: Biotechnology, Consumer Packaged Goods, Pharma

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