DNA Arrays

Methods and Protocols

  • Jang B. Rampal

Part of the Methods in Molecular Biology™ book series (MIMB, volume 170)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Edwin M. Southern
    Pages 1-15
  3. Jordanka Zlatanova, Andrei Mirzabekov
    Pages 17-38
  4. Radoje Drmanac, Snezana Drmanac
    Pages 39-51
  5. Glenn H. McGall, Jacqueline A. Fidanza
    Pages 71-101
  6. Christian Jurinke, Dirk van den Boom, Charles R. Cantor, Hubert Köster
    Pages 103-116
  7. Patrick Cooley, Debra Hinson, Hans-Jochen Trost, Bogdan Antohe, David Wallace
    Pages 117-129
  8. David W. Galbraith, Jiří Macas, Elizabeth A. Pierson, Wenying Xu, Marcela Nouzová
    Pages 131-140
  9. Ulrich Certa, Antoine de Saizieu, Jan Mous
    Pages 141-156
  10. Rogelio Maldonado-Rodriguez, Kenneth L. Beattie
    Pages 157-171
  11. Radoje Drmanac, Snezana Drmanac, Joerg Baier, Gloria Chui, Dan Coleman, Robert Diaz et al.
    Pages 173-179
  12. Muhammad Sohail, Edwin M. Southern
    Pages 181-199
  13. Sarah H. Haddock, Christine Quartararo, Patrick Cooley, Dat D. Dao
    Pages 201-210
  14. Ralph Sinibaldi, Catherine O’Connell, Chris Seidel, Henry Rodriguez
    Pages 211-222
  15. Peter Kalocsai, Soheil Shams
    Pages 223-236
  16. Arthur E. Dixon, Savvas Damaskinos
    Pages 237-246
  17. Kenneth E. Rubenstein
    Pages 247-256
  18. Back Matter
    Pages 257-264

About this book

Introduction

Microarray technology, which permits the rapid, simultaneous, and highly sensitive analysis of large numbers of biological samples, is now coming into widespread use for advanced research on gene expression, mutation analysis, proteomics, and gene sequencing. In DNA Arrays: Methods and Protocols, Jang Rampal and a authoritative panel of researchers, engineers, and technologists explain in detail how to design and construct these DNA arrays, as well as how to hybridize them with biological samples for analysis. In step-by-step instructions these experts detail not only how to attach or print arrays on various matrices, but also biological sample preparation (DNA and RNA), hybridization conditions, signal detection, probe optimization, different printing technologies, and data collection and analysis (bioinformatics). Additional topics covered include genotyping, sequencing by hybridization, antisense reagents, HLA-DQA typing techniques, and gene expression analysis. Rounding out the technical presentation are three chapters that review the history of microarrays, the ethical ramifications of genetic analysis using DNA arrays, and the business aspects of biochip technologies. Forward-looking and state-of-the-art, DNA Arrays: Methods and Protocols provides all investigators engaged in biological and biomedical research the full range of effective, readily reproducible microarray techniques needed today to analyze on a large scale the many different genes and gene sequences now available from the Human Genome Project.

Editors and affiliations

  • Jang B. Rampal
    • 1
  1. 1.Beckman Coulter, Inc.Fullerton

Bibliographic information

  • DOI https://doi.org/10.1385/1592592341
  • Copyright Information Humana Press 2001
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-822-6
  • Online ISBN 978-1-59259-234-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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