Epstein-Barr Virus Protocols

  • Joanna B. Wilson
  • Gerhard H. W. May

Part of the Methods in Molecular Biology™ book series (MIMB, volume 174)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Genome and Transcript Analyses

  3. Viral Detection

    1. Front Matter
      Pages 77-77
    2. Gerald Niedobitek, Hermann Herbst
      Pages 79-91
    3. Gregory J. Babcock, Emily M. Miyashita-Lin, David A. Thorley-Lawson
      Pages 103-110
    4. Lisa L. Decker, Gregory J. Babcock, David A. Thorley-Lawson
      Pages 111-116
  4. Culture Methods

    1. Front Matter
      Pages 117-117
    2. Lindsey M. Hutt-Fletcher, Susan M. Turk
      Pages 119-123
    3. Teresan Frisan, Victor Levitsky, Maria Masucci
      Pages 125-127
    4. Dieter Kube, Martina Vockerodt
      Pages 155-164
    5. Christopher W. Dawson, Lawrence S. Young
      Pages 165-172
    6. Christopher W. Dawson, Lawrence S. Young
      Pages 173-180
    7. Neil A. Jones, Caroline Dive
      Pages 181-187
  5. Immune Assays

    1. Front Matter
      Pages 197-197
    2. Teresa Frisan, Victor Levitsky, Maria Masucci
      Pages 199-201
    3. Victor Levitsky, Teresa Frisan, Maria Masucci
      Pages 203-208
    4. Victor Levitsky, Teresa Frisan, Maria Masucci
      Pages 209-211
    5. Teresa Frisan, Victor Levitsky, Maria Masucci
      Pages 213-216
  6. Viral Protein Detection

    1. Front Matter
      Pages 217-217
    2. Martin Rowe, Anja Mehl
      Pages 219-227
    3. Martin Rowe, Matthew Jones
      Pages 229-242
  7. Protein-Protein, Protein-DNA, and Protein-RNA Interactions

    1. Front Matter
      Pages 247-247
    2. Lothar J. Strobl, Ursula Zimber-Strobl
      Pages 271-277
    3. Qin Zhang, Eduardo A. Montalvo
      Pages 279-287
    4. Yi-Chun James Wang, Eduardo A. Montalvo
      Pages 289-296
    5. Kenneth G. Laing, Androulla Elia, Ian W. Jeffrey, Michael J. Clemens
      Pages 297-310
  8. Protein Activity Assays

    1. Front Matter
      Pages 311-311
    2. Olivier Gires, Marius Ueffing, Wolfgang Hammerschmidt
      Pages 313-323
  9. Tissue and In Vivo Protocols

    1. Front Matter
      Pages 345-345
    2. Vicki Save, Philip J. Coates, Peter A. Hall
      Pages 347-359
    3. Joanna B. Wilson, Mark E. Drotar
      Pages 361-377
    4. John Curran
      Pages 379-389

About this book


The application of recombinant DNA technology to the analysis of Epstein-Barr virus (EBV) is rapidly developing sufficient insight into the virus-host interaction, so that its role in disease pathology is now often discernible and can increasingly be interdicted. In Epstein-Barr Virus Protocols, Joanna Wilson and Gerhard May have assembled a collection of the key molecular biology protocols used in the analysis of Epstein-Barr virus, along with a series of valuable immunology, cell biology, and transgenic mouse protocols. Described in step-by-step detail by experts who use them regularly, these readily reproducible techniques include methods for gene expression with mini-EBV plasmids, for expression analysis by FISH, for EBV detection and quantitation, and for cell proliferation and death assays. In addition, the authors provide information on EBV-based vectors, an up-to-date map of EBV, a comprehensive table of available latent protein antisera, and assays from in vitro to cell to organ to organism levels.
Timely and highly practical, Epstein-Barr Virus Protocols provides powerful tools for elucidating the life cycle of EBV and its host interactions, work that promises the emergence of major new treatments and cures for EBV-associated diseases, including several forms of human cancer.

Editors and affiliations

  • Joanna B. Wilson
    • 1
  • Gerhard H. W. May
    • 2
  1. 1.I.B.L.S. Division of Molecular GeneticsUniversity of GlasgowUK
  2. 2.I.B.L.S. Division of Biochemistry and Molecular BiologyUniversity of GlasgowUK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2001
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-690-1
  • Online ISBN 978-1-59259-227-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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