Gene Knockout Protocols

  • Martin J. Tymms
  • Ismail Kola

Part of the Methods in Molecular Biology book series (MIMB, volume 158)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Paul J. Hertzog, Ismail Kola
    Pages 1-10
  3. Vivienne S. Marshall, Michelle A. Waknitz, James A. Thomson
    Pages 11-18
  4. Thomas M. DeChiara
    Pages 19-45
  5. Lino Tessarollo
    Pages 47-63
  6. Louise D. Barnett, Frank Köntgen
    Pages 65-82
  7. Trevor J. Wilson, Ismail Kola
    Pages 83-94
  8. Mireille H. Lahoud, Trevor J. Wilson, Paul J. Hertzog
    Pages 107-119
  9. Angelika Bonin, Susan W. Reid, Lino Tessarollo
    Pages 121-134
  10. Mika Tanaka, Anna-Katerina Hadjantonakis, Andras Nagy
    Pages 135-154
  11. Roderick T. Bronson
    Pages 155-180
  12. Ivan Bertoncello, Brenda Williams
    Pages 181-203
  13. L. Philip Sanford, Suhas Kallapur, Ilona Ormsby, Thomas Doetschman
    Pages 217-225
  14. Gabriel A. Quinlan, Bruce P. Davidson, Patrick P. L. Tam
    Pages 227-250
  15. Hein te Riele, Conny Brouwers, Marleen Dekker
    Pages 251-262
  16. Francesca Lazner, Ismail Kola, Elizabeth Stadler, Andrew G. Elefanty
    Pages 287-300
  17. Linda M. Whyatt, Peter D. Rathjen
    Pages 301-318
  18. Sika Ristevski
    Pages 319-334
  19. Emma Whitelaw, Heidi Sutherland, Margot Kearns, Hugh Morgan, Linda Weaving, David Garrick
    Pages 351-368
  20. John J. Sharp, Carol C. Linder, Larry E. Mobraaten
    Pages 381-396
  21. Jillian M. Shaw, Magosaburo Kasai
    Pages 397-419
  22. Back Matter
    Pages 421-431

About this book


As the major task of sequencing the human genome nears completion and the full complement of human genes are catalogued, the task of understanding the normal biological functions of genes and how their alteration leads to diseased states becomes more imperative. In Gene Knockout Protocols, highly skilled investigators with extensive experience in gene targeting and mouse genetics describe their best techniques for the design of targeting constructs and for genetic phenotype analysis. These proven methods contain step-by-step instructions, as well as notes on pitfalls to avoid, and emphasize techniques that are relevant to researchers carrying out gene targeting work. These include embryo transplantation, in vitro embryonic stem cell differentiation, creation of aggregation chimeras, mouse pathology, embryo cryopreservation, and transplantation. Issues such as the use of existing mouse mutation resources and the influence of genetic background and epigenetic effects upon phenotype are also covered.
State-of-the-art and highly practical, Gene Knockout Protocols not only constitutes an invaluable source of readily reproducible techniques for those just entering the field of gene targeting, but also a key reference for all genetic researchers today.

Editors and affiliations

  • Martin J. Tymms
    • 1
  • Ismail Kola
    • 2
  1. 1.National Vision Research Institute of AustraliaCarltonAustralia
  2. 2.Centre for Functional Genomics and Human Disease, Institute of Reproduction and DevelopmentMonash UniversityClaytonAustralia

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2001
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-572-0
  • Online ISBN 978-1-59259-220-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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