© 2001

Protocols for Neural Cell Culture

  • Sergey Fedoroff
  • Arleen Richardson

Part of the Springer Protocols Handbooks book series (SPH)

Table of contents

  1. Front Matter
    Pages i-xxii
  2. Valérie Castellani, Jürgen Bolz
    Pages 1-12
  3. Dominique Muller, Nicolas Toni, Pierre-Alain Buchs, Lorena Parisi, Luc Stoppini
    Pages 13-27
  4. Carol A. Colton, Meggan Czapiga, Toby N. Behar
    Pages 29-37
  5. Bernard Rogister, Gustave Moonen
    Pages 39-48
  6. Bernhard H. J. Juurlink
    Pages 59-69
  7. Mary I. Johnson
    Pages 71-94
  8. Mary I. Johnson, Richard P. Bunge, Patrick M. Wood
    Pages 95-115
  9. V. Wee Yong, Jack P. Antel
    Pages 129-138
  10. Bernhard H. J. Juurlink, Shawn K. Thorburne, Richard M. Devon
    Pages 149-160
  11. Ronald Doucette
    Pages 161-171
  12. Angela Gritti, Rossella Galli, Angelo Luigi Vescovi
    Pages 173-197
  13. Paul Honegger, Florianne Monnet-Tschudi
    Pages 199-218
  14. Kaoru Murayama, Natalia N. Singh, Angela Helmrich, David W. Barnes
    Pages 219-228
  15. Sonia N. Yeung, Frank Tufaro
    Pages 229-244
  16. Paul J. Price, Gregory J. Brewer
    Pages 255-264

About this book


In Protocols for Neural Cell Culture, Third Ed., Sergey Fedoroff and Arleen Richardson extensively revise, update, and expand their best-selling and highly praised collection of readily reproducible neural tissue culture protocols. This 3rd edition adds 11 chapters describing important new procedures for the isolation, growth, and characterization of neural stem cells and for the manipulation of glial progenitor cells, as well as essential procedures for hippocampal and microglial slice cultures and transfection of neurons in culture with adenovirus. It includes key techniques for the preparation of substrata, the use of serum-free media, maintaining hybridomas, and the production and purification of monoclonal antibodies. For scientists not trained in neuroanatomy, but faced with dissecting the brain and spinal cord, most chapters in the 3rd edition provide fully detailed dissection procedures.
Protocols for Neural Cell Culture, Third Ed. is a richly augmented updating of the tried and tested laboratory procedures that have made earlier editions an indispensable reference and guide to neural cell culture. Its unique wealth of practical detail on a wide range of tissue culture systems having many applications ensure that this new edition will remain an essential resource for all investigators using cell culture methodology in studying the brain and its disorders.


brain central nervous system myelin nervous system neurons neuroscience

Editors and affiliations

  • Sergey Fedoroff
    • 1
  • Arleen Richardson
    • 1
  1. 1.Department of Anatomy and Cell Biology, School of MedicineUniversity of SaskatchewanSaskatoonCanada

Bibliographic information

  • Book Title Protocols for Neural Cell Culture
  • Editors Sergey Fedoroff
    Arleen Richardson
  • Series Title Springer Protocols Handbooks
  • Series Abbreviated Title Springer Protocols Handbooks
  • DOI
  • Copyright Information Humana Press 2001
  • Publisher Name Humana Press
  • eBook Packages Springer Book Archive
  • Softcover ISBN 978-0-89603-902-5
  • eBook ISBN 978-1-59259-207-4
  • Series ISSN 1949-2448
  • Series E-ISSN 1949-2456
  • Edition Number 3
  • Number of Pages XXII, 262
  • Number of Illustrations 0 b/w illustrations, 0 illustrations in colour
  • Topics Neurosciences
  • Buy this book on publisher's site
Industry Sectors
Health & Hospitals


"In addition to covering standard protocols in well-established culture systems, this is one of the first manuals to include a chapter on protocols to culture neural stem cells. Another unique feature is the inclusion of pictures to facilitate the dissection of particular brain areas, which will be of great help for workers not completely familiar with different aspects of developmental neuroanatomy. . .This is a valuable tool for "culturists" of neural cells. This edition updates protocols for the culturing of neural cell types traditionally used in laboratories studying different aspects of neurobiology and it provides easy-to follow instructions for setting up and maintaining cultures of progenitor cells that will be used in many laboratories in years to come."-Doody's Health Science Book review Journal

"...presents a series of excellent neural tissue culture protocols designed for bench use."
-Trends in Cell Biology

"...a valuable resource that will find its place on many laboratory shelves..."
-Trends in Biotechnology

"...the recipes are comprehensive and easy to follow."
-Trends in Neurosciences

"...indispensable for every project in neuroscience."
-Doody's Health Sciences Book Review Journal