© 2002

Apoptosis Techniques and Protocols

  • Andréa C. LeBlanc

Part of the Neuromethods book series (NM, volume 37)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Douglas W. Ethell, Douglas R. Green
    Pages 21-34
  3. Younes Bounhar, Omar Tounekti, Andréa C. LeBlanc
    Pages 35-59
  4. Kathleen A. Maguire-Zeiss, William J. Bowers, Howard J. Federoff
    Pages 61-82
  5. P. Scott Eastman, Jeanne F. Loring
    Pages 107-132
  6. H. Michael Tucker, Steven Estus
    Pages 133-147
  7. Barbara Karten, Bronwyn L. MacInnis, Hubert Eng, Yoko Azumaya, Grace Martin, Karen Lund et al.
    Pages 163-175
  8. Greg M. Cole, Karen Gylys
    Pages 177-203
  9. Kevin A. Roth
    Pages 205-224
  10. Arun K. Raina, Lawrence M. Sayre, Craig S. Atwood, Catherine A. Rottkamp, Ayala Hochman, Xiongwei Zhu et al.
    Pages 225-246
  11. Back Matter
    Pages 247-250

About this book


The study of the apoptotic process has grown exponentially since the publication of the first edition of Apoptosis Techniques and Protocols for the Neuromethods series in 1997. In this greatly updated second edition, seasoned experts describe in step-by-step detail their best state-of-the-art techniques for studying neuronal cell death. These readily reproducible methods solve a wide variety of research problems, including the detection of the key proteins involved in neuronal apoptosis (Bax protein, cytochrome-c, and caspases), the direct assessment of the role of pro-apoptotic proteins in neurons by viral infections and microinjections, and the detection of proapoptotic proteins in situ. There are also hands-on methods for the study of apoptosis mechanisms in neuronal compartments, for studying synaptosis, and for establishing gene expression profiles in neurodegenerative brain tissues by using DNA microarrays. Written by investigators who have used the techniques extensively, each protocol includes tips on avoiding pitfalls, notes on the method's advantages and disadvantages, and a critical survey of the literature.
Cutting-edge and highly practical, Apoptosis Techniques and Protocols, Second Edition, offers both novice and seasoned investigators a rich panoply of the productive tools they need to unravel the molecular mechanisms of neuronal cell death.

Editors and affiliations

  • Andréa C. LeBlanc
    • 1
  1. 1.Bloomfield Center for Research in Aging, Lady Davis Institute for Medical Research, Jewish General Hospital and Department of Neurology and NeurosurgeryMcGill UniversityMontrealCanada

Bibliographic information

  • Book Title Apoptosis Techniques and Protocols
  • Editors Andréa C. LeBlanc
  • Series Title Neuromethods
  • DOI
  • Copyright Information Humana Press 2002
  • Publisher Name Springer, Totowa, NJ
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-58829-012-0
  • Softcover ISBN 978-1-61737-306-0
  • eBook ISBN 978-1-59259-188-6
  • Series ISSN 0893-2336
  • Series E-ISSN 1940-6045
  • Edition Number 2
  • Number of Pages XV, 250
  • Number of Illustrations 0 b/w illustrations, 0 illustrations in colour
  • Topics Neurosciences
  • Buy this book on publisher's site
Industry Sectors
Health & Hospitals


Review of the first edition:
"The book is useful for an actively working neuroscientist who is going to study apotosis and has enough experience to choose the most suitable models and protocols among all the diverse offerings of the book." Three Stars. -Doody's Health Sciences Book Review Journal

"...the book has updated many of the state-of-the-art techniques for analysis of neuronal apoptosis. More importantly, the book also offers the first-hand and step-by-step experimental details for molecular, cellular, biochemical and immunohistochemical assays of neuronal apoptotic death." - Biotechnic & Histochemistry