Calcium-Binding Protein Protocols

Volume 1: Reviews and Case Studies

  • Hans J. Vogel

Part of the Methods in Molecular Biology™ book series (MIMB, volume 172)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Introduction and Reviews

    1. Front Matter
      Pages 1-1
    2. Hans J. Vogel, Richard D. Brokx, Hui Ouyang
      Pages 3-20
    3. Robert J.P. Williams
      Pages 21-49
    4. Zongchao Jia, Christopher M. Hosfield, Peter L. Davies, John S. Elce
      Pages 51-67
    5. Claus W. Heizmann
      Pages 69-80
    6. Harold M. Farrell Jr., Thomas F. Kumosinski, Edyth L. Malin, Eleanor M. Brown
      Pages 97-140
  3. Calcium-Binding Proteins: Case Studies

    1. Front Matter
      Pages 141-141
    2. Raymond E. Zielinski
      Pages 143-149
    3. John A. Putkey, Wen Liu
      Pages 151-160
    4. Joyce R. Pearlstone, Lawrence B. Smillie
      Pages 161-174
    5. Jean Christophe Deloulme, Gaëlh Ouengue Mbele, Jacques Baudier
      Pages 185-198
    6. Jean-René Alattia, Kit I Tong, Masatoshi Takeichi, Mitsuhiko Ikura
      Pages 199-210
    7. Katsutoshi Nitta
      Pages 211-224
    8. Hyoung-Min Kang, Nolan R. Filipenko, Geetha Kassam, David M Waisman
      Pages 225-234
    9. Senadhi Vijay-Kumar, Vinod D. Kumar
      Pages 261-279
    10. ChulHee Kang, William R. Trumble, A. Keith. Dunker
      Pages 281-294
    11. Josep Rizo, Josep Ubach, Jesús GarcÍa
      Pages 305-316
    12. Seun-Ah Yang, Claude Klee
      Pages 317-334
  4. Back Matter
    Pages 335-339

About this book


Calcium-binding proteins play an important role in a variety of vital biological processes, ranging from blood clotting and signal transduction in cells, to attaching proteins to membranes and serving as an integral source of calcium. In Calcium-Binding Protocols-Volume 1: Reviews and Case Studies and Volume 2: Methods and Techniques-Hans Vogel and a panel of leading researchers review the protein chemistry and behavior of this significant protein class, and provide a comprehensive collection of proven experimental techniques for their study both in vitro and in vivo. This first volume discusses the role of calcium in intracellular secondary messenger activation mechanisms, including unique aspects of calcium chemistry and its utilization in dairy proteins and blood clotting. Detailed case studies provide a wealth of valuable information about protein purification and characterization strategies, X-ray crystallography, and specific calcium-binding proteins and their modes of action. The second companion volume, Methods and Techniques, focuses on cutting-edge experimental methods for studying solution structure, stability, dynamics, calcium-binding properties, and biological activity of calcium-binding proteins in general.
Comprehensive and highly practical, the two volumes of Calcium-Binding Protocols provide experimental and clinical biologists with a host of advanced experimental methods that can be applied successfully to the study of both existing and newly discovered members of this critically important class of proteins.


Amino acid Calcium chemistry membrane protein protein family proteins

Editors and affiliations

  • Hans J. Vogel
    • 1
  1. 1.Department of Biological SciencesUniversity of CalgaryCalgaryCanada

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2002
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-688-8
  • Online ISBN 978-1-59259-183-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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