Liposome Methods and Protocols

  • Subhash C. Basu
  • Manju Basu

Part of the Methods in Molecular Biology book series (MIMB, volume 199)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Physical Properties

    1. Front Matter
      Pages 1-1
    2. Massimo Masserini, Paola Palestini, Marina Pitto, Vanna Chigorno, Sandro Sonnino
      Pages 17-27
  3. Liposome Fusion/Modulation

    1. Front Matter
      Pages 29-29
    2. Eve-Isabelle Pécheur, Dick Hoekstra
      Pages 31-48
    3. Sujoy Ghosh, Robert Bell
      Pages 49-60
    4. Anu Puri, Maite Paternostre, Robert Blumenthal
      Pages 61-81
  4. Application of Liposomes

    1. Front Matter
      Pages 83-83
    2. Roger K. Bretthauer, Dennis W. Welsh
      Pages 131-143
    3. William L. Stone, Shyamali Mukherjee, Milton Smith, Salil K. Das
      Pages 145-161
    4. Debi P. Sarkar, Komal Ramani, Sandeep K. Tyagi
      Pages 163-173
    5. Sriram Neelamegham, Khushi L. Matta
      Pages 175-191
  5. Back Matter
    Pages 245-249

About this book


In vitro utilization of liposomes holds great potential as a powerful tool for drug targeting, gene transport across plasma and nuclear membranes, and enzyme therapy for patients with genetic disorders. In Liposome Methods and Protocols, a panel of skilled researchers describes in step-by-step detail their best laboratory techniques for elucidating liposome biology and realizing this promise. With these readily reproducible methods, investigators can illuminate such critical questions as the attachment of liposomes to cell surfaces, the permeation of liposomes through the plasma membranes, and the stability of liposomes in cellular and nuclear matrices. Each detailed protocol is presented by an investigator who is intimately familiar with its problems and capabilities, and includes a detailed list of materials to be used, troubleshooting tips straight from the benchtop, and notes on pitfalls to avoid. The emphasis is on clearly presenting the technical steps critical to experimental success that are too often omitted from methods published in the primary literature.
State-of-the-art and highly practical, Liposome Methods and Protocols provides an indispensable collection of proven techniques and references for researchers across many fields, including glycoproteins, glycolipids, glycosyltransferases, drug transport, viral transport, antibody delivery, synthetic peptide delivery to cells, and protease delivery.

Editors and affiliations

  • Subhash C. Basu
    • 1
  • Manju Basu
    • 1
  1. 1.Department of Chemistry and BiochemistryUniversity of Notre DameNotre Dame

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2002
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-845-5
  • Online ISBN 978-1-59259-175-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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