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Interleukin Protocols

Part of the book series: Methods in Molecular Medicine (MIMM, volume 60)

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Table of contents (36 protocols)

  1. Assaying Interleukins In Particular Pathologies

    1. Front Matter

      Pages 205-205
    2. Assaying Interleukin-6 in Breast Cancer

      • Judith Harmey, Amanda Haverty, Deirdre Foley, David Bouchier-Hayes
      Pages 207-216
    3. Cytokines, Stress, and Depressive Illness

      • Hymie Anisman, Shawn Hayley, Arun V. Ravindran, Zul Merali, Jenna Griffiths
      Pages 217-238
    4. Measurement of Interleukins in Cutaneous Disorders

      • Khusru Asadullah, Antje Haeussler-Quade, Wolf Dietrich Doecke, Wolfram Sterry, Hans-Dieter Volk
      Pages 239-250
    5. Interleukins in Graves′ Disease

      • Jan Komorowski
      Pages 251-261
    6. Evaluation of Cytokines in Migraine Patients by ELISA

      • Irene Munno, Mariarosaria Marinaro, Giuseppe Lacedra, Antonia Bassi, Vincenzo Centonze
      Pages 263-272
  2. Assaying Interleukins in Particular Biological Fluids

    1. Front Matter

      Pages 283-283
    2. Measurement of Cytokines in Peritoneal Fluids

      • Reijo Punnonen, Juha Punnonen
      Pages 285-291
    3. Measurement of Cytokines in Seminal Plasma

      • Marek Glezerman, Eitan Lunenfeld, Mahmoud Huleihel
      Pages 293-304
    4. Measurement of Cytokines in Induced Sputum

      • Peter G. Gibson, Jodie L. Simpson
      Pages 305-312
    5. Cytokines in Blister Fluids

      • Franco Ameglio, Luciano D’Auria
      Pages 313-325
    6. Measurement of Cytokines in Synovial Fluid

      • Fabrizio De Benedetti
      Pages 327-335
    7. Measurement of Cytokines in Cerebrospinal Fluid

      • Toshiyuki Yokoyama
      Pages 337-344
    8. Detection of Cytokines in Tears

      • Archana Thakur, Mark D. P. Willcox
      Pages 345-353
  3. Newer Techniques: Interleukin Signal Transduction,

    1. Front Matter

      Pages 355-355
    2. Applications for Green Fluorescent Protein in Cell Signaling

      • Paul Brennan, Emmanuelle Astoul
      Pages 385-391
    3. Use of Pichia pastoris for Production of Recombinant Cytokines

      • Kevin P. Murphy, Csaba Pazmany, Mark D. Moody
      Pages 393-407

About this book

Interleukins are a family of proteins that regulate the maturation, diff- entiation, or activation of cells involved in immunity and inflammation, and belong to a broader family termed cytokines. Collectively these proteins are the key orchestrators of host defense and the response to tissue injury. There are currently 23 different interleukins (numbered from IL-1 to IL-23), although the full extent of the interleukin family will only become clear upon analysis of the human genome sequence. Most important, interleukins are central to the pathogenesis of a wide range of diseases that involve an immune com- nent, including such conditions as rheumatoid arthritis, multiple sclerosis, ulcerative colitis, psoriasis, and asthma. Interleukins have also been imp- cated in other conditions, including cancer, migraine, myocardial infarction, and depression. In essence, when cells are activated by interleukins, a program of gene expression is initiated in the target cell that alters the cell’s phenotype, leading to enhanced immune reactivity, inflammation, and/or proliferation. Interleukins are therefore at the core of the cellular basis for many diseases. They are the subject of intense investigation by biomedical researchers and the targeting or use of interleukins in the clinic is proceeding apace. Approaches such as t- geting IL-4 in asthma or IL-1 in joint disease are being pursued, and it is likely that in the next 5–10 years a number of new therapies based on either inhib- ing or administering interleukins will be available.

Reviews

"this volume offers protocols for measurement of various interleukins. Most are of sufficient detail so as to serve as a laboratory guide for investigators. Investigators with a specific interest in analyzing a given interleukin will find this volume helpful. "-Doody's Health Sciences Book Review Journal

"Comprehensive and highly practical, Interleukin Protocols offers biomedical investigators a stellar collection of all the major techniques needed to analyze the role of interleukins in disease, to improve diagnosis, and to foster the rapid emergence of new and more powerful therapeutics." - Journal of Investigational Allergology and Clinical immunology

"A variety of techniques is represented in 36 chapters which are written in a concise, uniform format b y authors who obviously have a lot of hands-on experience with the methods covered...If you are working in this area, put this book on a shelf in the lab and it will not be just recently started graduate students who will gracefully turn to it. - Chembiochem

"The protocols that are described in the book are user friendly, in the sense that they are not only well detailed and easy to follow but also reliable. The researchers that are naive in the field will find a lot of useful information in the book whereas the experienced ones will be able to use the book as a source of additional information on the methods already in use. A must therefore for the psychoneuroendocrinologist with a working interest in the relationships between the (neuroendocrine) brain and the immune system." -Psychoneuroendocriniology

Editors and Affiliations

  • Department of Biochemistry and Biotechnology Institute, Trinity College, Dublin, Ireland

    Luke A. J. O’Neill

  • Department of Pharmacology, Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Dublin, Ireland

    Andrew Bowie

Bibliographic Information

  • Book Title: Interleukin Protocols

  • Editors: Luke A. J. O’Neill, Andrew Bowie

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1385/1592591469

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2001

  • Hardcover ISBN: 978-0-89603-738-0Published: 12 July 2001

  • Softcover ISBN: 978-1-61737-162-2Published: 09 November 2010

  • eBook ISBN: 978-1-59259-146-6Published: 02 February 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: XV, 443

  • Topics: Immunology

  • Industry Sectors: Biotechnology, Consumer Packaged Goods, Health & Hospitals, Pharma

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access