Renal Cancer

1. In Vivo Experimental Techniques

   1. Angiogenesis Assays

      • Jack H. Mydlo

      Pages 265-275

   2. Antiangiogenic Approaches to Renal Cell Carcinoma

      • Steven C. Campbell, Frank K. Reiher, Norman D. Smith Jr.

      Pages 277-298

2. Clinical Trials and Experiments

   1. Front Matter

      Pages 299-299

      PDF

   2. Interferon Alpha, GM-CSF-Activated T Cells, and IL-6 in Renal Cell Carcinoma

      • Belur Patel, Arie Belldegrun

      Pages 301-312

   3. Immunotherapeutic Approach to Cancer with Cutaneous DNA Vaccination

      • Wilbur B. Bowne, William G. Hawkins, Jonathan J. Lewis

      Pages 313-321

   4. Monoclonal Antibodies

      • Egbert Oosterwijk

      Pages 323-341

   5. Treatment Strategies in Metastatic Renal Cell Carcinoma

      • Peter F. Mulders

      Pages 343-359

   6. Assessment of T-Cell Immune Dysfunction in Patients with Renal Cell Carcinoma

      • Robert G. Uzzo, Vladimir Kolenko, Andrew C. Novick, James H. Finke

      Pages 361-376

   7. Transforming Growth Factor-λ1 as a Novel Marker of Response to Therapy for Renal Cell Carcinoma

      • Howard L. Adler

      Pages 377-384

   8. Static and Flow Cytometry

      • Ralph Madeb, Dov Pode, Ofer Nativ

      Pages 385-399

3. Erratum to: Immunotherapeutic Approach to Cancer with Cutaneous DNA Vaccination

   • Wilbur B. Browne, William G. Hawkins, Jonathan J. Lewis

   Pages E1-E1

4. Back Matter

   Pages 401-403

   PDF

The purpose of Renal Cancer: Methods and Protocols is to introduce the surgeon, clinician, investigator, and research scientist to the basic methods employed in the diagnosis and treatment of renal cancer. Treatment of localized renal cancer is surgical. Treatment of metastasis with resection, radiotherapy, chemotherapy, and immunotherapy has had limited results. Therefore, new avenues of treatment are necessary. In planning this work, I have attempted to incorporate coverage by specialists from a variety of disciplines, each applying their individual expertise in renal cancer therapy. Though many of the participating authors are urologists, there are also valuable contributions from medical oncologists, laboratory inv- tigators, and pathologists. One of the inherent problems in publishing a book that describes molecular techniques, especially those techniques that are rapidly evolving, is that some of these may become obsolete after a few years. However, many of the procedures detailed here are standard approaches that have already wi- stood the test of time. Reverse transcriptase PCR, or RT-PCR, a technique that produces m- tiple copies of selected sequences of DNA, has a sensitivity of a millionfold amplication of a single cell, and thus constitutes a very precise technique for measuring the presence of tumor cells. By combining this technique with genomic hybridization, cadherin,ß and metalloproteinase expression we may then reveal other important factors in the detection, staging, aggressiveness, and treatment of this disease.

"Virtually all potentially relevant in vitro and in vivo methodologies are addressed in this book. The sections describing the respective techniques are well written and stylistically equivalent...This book will be a useful addition to most institutional libraries. It will also be of some value to experienced investigators who already possess hands-on familiarity with a particular technique, but merely wish to adapt such methodology to renal cell cancer systems." -Doody's Health Sciences Book Review Journal

"...the intros are great! The writing style is surprisingly uniform and very readable. There is plentiful reference to clinical reality and a strong emphasis on novel treatments." -ACP News

"...the book is well written and the experimental protocols that were included are described in great detail." - Clinical Immunology

• Temple University School of Medicine, Philadelphia

  Jack H. Mydlo

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