© 2001

Nonviral Vectors for Gene Therapy

Methods and Protocols

  • Mark A. Findeis

Part of the Methods in Molecular Medicine™ book series (MIMM, volume 65)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Takuro Niidome, Haruhiko Aoyagi
    Pages 11-21
  3. Gert W. Bos, Daan J. A. Crommelin, Wim E. Hennink
    Pages 35-60
  4. David Oupicky, Martin L. Read, Thierry Bettinger
    Pages 61-78
  5. Fuxing Tang, Jeffrey A. Hughes
    Pages 79-88
  6. Erik T. Rump, Erik A. L. Biessen, Theo J. C. van Berkel, Martin K. Bijsterbosch
    Pages 89-104
  7. Carole Neves, Daniel Scherman, Pierre Wils
    Pages 105-109
  8. Bo-Hua Zhong, George Y. Wu, Catherine H. Wu
    Pages 111-121
  9. Yoshiyuki Takei, Atsushi Maruyama, Toshihiro Akaike, Nobuhiro Sato
    Pages 123-129
  10. Lonnie D. Shea, David J. Mooney
    Pages 195-207
  11. S. Dean Allison, Thomas J. Anchordoquy
    Pages 225-252
  12. Chad S. Braun, Lisa A. Kueltzo, C. Russell Middaugh
    Pages 253-284

About this book


Nonviral gene therapy-an emerging field with great clinical promise-avoids many problems associated with viral gene therapy and offers the possibility of superior clinical effectiveness. In Nonviral Vectors for Gene Therapy: Methods and Protocols, Mark A. Findeis has assembled a panel of active researchers to present their best methods not only for preparing, handling, and characterizing gene delivery agents, but also for gene delivery. To help those preparing and characterizing gene transfer agents, the contributors examine a broad range of compounds that bind with DNA to form the compact complexes that facilitate cellular delivery-among them peptide conjugates, synthetic polymers, and lipids. They also outline specific approaches to gene transfer in vivo, including direct delivery by intratumoral injection and indirect delivery by cell-specific targeting of DNA complexes, and discuss in detail many spectroscopic techniques for characterizing nonviral gene delivery agents. Opportunities for the development and application of these novel vectors in the research lab and eventually, in the clinic, are highlighted.
Comprehensive and state-of-the-art, Nonviral Vectors for Gene Therapy: Methods and Protocols illuminates for today's investigators the powerful new approaches to the creation of nonviral materials, as well as their efficacious use in delivering therapeutic genes to patients across the spectrum of human disease.

Editors and affiliations

  • Mark A. Findeis
    • 1
  1. 1.Praecis Pharmaceuticals IncorporatedWaltham

Bibliographic information

  • Book Title Nonviral Vectors for Gene Therapy
  • Book Subtitle Methods and Protocols
  • Editors Mark A. Findeis
  • Series Title Methods in Molecular Medicine™
  • DOI
  • Copyright Information Humana Press 2001
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-0-89603-712-0
  • Softcover ISBN 978-1-61737-145-5
  • eBook ISBN 978-1-59259-139-8
  • Series ISSN 1543-1894
  • Series E-ISSN 1940-6037
  • Edition Number 1
  • Number of Pages XI, 399
  • Number of Illustrations 0 b/w illustrations, 0 illustrations in colour
  • Topics Cell Biology
  • Buy this book on publisher's site
Industry Sectors
Health & Hospitals


"The book begins with a series of chapters covering specific protocols concerning gene delivery agents. These are followed by several review chapters that provide additional information on preparing and characterizing gene transfer agents. Particular focus is given to spectroscopic techniques. The final chapter provides a review of renal gene therapy. Chapters are well organized and often accompanied by helpful figures. Each chapter includes a list of references, an an index is provided. Contributors are from research facilities throughout the world. This book is highly recommended for collections serving researchers in the area of gene therapy." - E-Streams