© 2001

Colorectal Cancer

Methods and Protocols

  • Steven M.

Part of the Methods in Molecular Medicine book series (MIMM, volume 50)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Christopher A. Moskaluk
    Pages 1-13
  3. Jeffrey C. Harper, Reid B. Adams, Steven M. Powell
    Pages 21-24
  4. Wa′el El-Rifai, Sakari Knuutila
    Pages 25-33
  5. Svetlana D. Pack, Zhengping Zhuang
    Pages 35-50
  6. Darryl Shibata
    Pages 59-67
  7. Yann R. Parc, Kevin C. Halling
    Pages 69-79
  8. Julie M. Cunningham, David J. Tester, Stephen N. Thibodeau
    Pages 87-98
  9. William M. Grady, Lois L. Myeroff, Hongmei He, Sanford Markowitz
    Pages 99-111
  10. Lauri A. Aaltonen, Stina Roth
    Pages 167-174
  11. Lauri A. Aaltonen, Egle Avizienyte
    Pages 175-183
  12. Lauri A. Aaltonen, Stina Roth, Charis Eng
    Pages 185-193
  13. Wa’el El-Rifai, Sakari Knuutila
    Pages 195-203

About this book


Colorectal cancer is a common and highly lethal cancer once spread whose treatment is improving as we gain a better understanding of its development. In Colorectal Cancer: Methods and Protocols, Steven M. Powell, MD, has assembled a hands-on collection of cutting-edge molecular methods for studying this solid tumor. These readily reproducible techniques range from gross microdissection of specimens to specific molecular analyses. Here both the novice and experienced researcher will find productive chromosomal analyses, including comparative genomic hybridization and FISH assays, and revealing gene expression analyses, including immunohistochemical assays, Western blotting, and microarray assays. Also presented are mutational analyses of colon tumors and blood samples to determine whether specific patients have inherited a significant predisposition to colorectal cancer. Each method is described in step-by-step detail by an established expert to ensure robust experimental results, and all are accompanied by detailed notes on pitfalls to avoid.
Eminently practical, with each technique presented as if the expert in the field who had perfected it were guiding the investigator, Colorectal Cancer: Methods and Protocols provides cancer investigators with a gold standard collection of all the key genetic, cellular, and molecular biological methods for analyzing colon cancer today.

Editors and affiliations

  • Steven M.
    • 1
  1. 1.University of Virginia Health SystemCharlottesville

Bibliographic information

  • Book Title Colorectal Cancer
  • Book Subtitle Methods and Protocols
  • Editors Steven M. Powell
  • Series Title Methods in Molecular Medicine
  • DOI
  • Copyright Information Humana Press 2001
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-0-89603-767-0
  • Softcover ISBN 978-1-61737-179-0
  • eBook ISBN 978-1-59259-084-1
  • Series ISSN 1543-1894
  • Series E-ISSN 1940-6037
  • Edition Number 1
  • Number of Pages XIII, 294
  • Number of Illustrations 0 b/w illustrations, 0 illustrations in colour
  • Topics Oncology
  • Buy this book on publisher's site
Industry Sectors
Health & Hospitals
Oncology & Hematology


"This is quite interesting and a valuable volume. It aims to instruct investigators in the key genetic, cellular and molecular biological methods for analyzing colorectal tumors. It's quite precise in its description of these techniques and, as the preface says, "The focused techniques and assays are described in sufficient detail to allow researchers to start an experiment on colon tumors and proceed from beginning to end as if the expert in the field who has performed these studies were guiding them at the bench." A large amount of practical information is packed into one small volume and this volume is ideal for students and fellows who wish to expand their knowledge in this area and have a ready volume reference in developing new techniques...The volume is also good value for those of us who don't get into the lab as it clarifies many of the techniques which are used and helps the non-involved reader to understand the terminology and techniques used in this important area of cancer research." - Cancer Forum

"...Dr. Saltz has produced as concise a presentation as possible within the framework of the widely scattered disciplines searching the complexity of diagnostic and therapeutic problems occurring among patients with colorectal cancer." - Comprehensive Therapy