Developmental Biology Protocols

1. Abnormal Development and Teratology

   1. Neural Tube Defects

      • Takayuki Inagaki, Jodi L. Smith, Marion L. Walker, Gary C. Schoenwolf

      Pages 161-166

   2. Experimental Manipulation and Morphometric Analysis of Neural Tube Development

      • Mary E. Desmond, Patricia A. Haas

      Pages 167-178

   3. Isolation of Neuroepithelium and Formation of Minispheres

      • Mary E. Desmond, Marcia C. Field

      Pages 179-184

   4. Examination of Normal and Abnormal Placentation in the Mouse

      • Michael R. Blackburn

      Pages 185-193

   5. Palatal Dysmorphogenesis

      • Barbara D. Abbott

      Pages 195-201

   6. Palatal Dysmorphogenesis

      • Gary A. Held, Barbara D. Abbott

      Pages 203-217

   7. Transspecies Grafting as a Tool to Understand the Basis of Murine Developmental Limb Abnormalities

      • Sheila M. Bell, Claire M. Schreiner, William J. Scott

      Pages 219-226

   8. Assessment of Laterality Defects in Rodent Embryos

      • Masahiko Fujinaga

      Pages 227-238

   9. Cardiac Morphogenesis and Dysmorphogenesis I. Normal Development

      • Andy Wessels, Roger Markwald

      Pages 239-259

   10. Analysis of Two Aspects of Left-Right Patterning of the Vertebrate Heart

       • Alvin J. Chin

       Pages 261-270

   11. Biologically Based Risk Assessment Models for Developmental Toxicity

       • Christopher Lau, R. Woodrow Setzer

       Pages 271-281

2. Screening and Mapping of Novel Genes and Mutations

   1. Front Matter

      Pages 283-495

      PDF

   2. Positional Cloning

      • Lin Xu, Yefu Li

      Pages 285-296

   3. Gene Trapping in Embryonic Stem Cells In Vitro to Identify Novel Developmentally Regulated Genes in the Mouse

      • Gary E. Lyons, Bradley J. Swanson, Melissa A. Haendel, Joshua Daniels

      Pages 297-307

   4. PCR-Based Cloning of Cortically Localized RNAs from Xenopus Oocytes

      • Jian Zhang, Mary Lou King

      Pages 309-314

   5. Analysis of mRNA Expression During Preimplantation Development

      • Keith E. Latham, Elena De La Casa, Richard M. Schultz

      Pages 315-331

   6. Differential Screens with Subtracted PCR-Generated cDNA Libraries from Subregions of Single Mouse Embryos

      • Akihiko Shimono, Richard R. Behringer

      Pages 333-344

   7. HPLC-Based mRNA Differential Display

      • Thomas B. Knudsen

      Pages 345-349

   8. Production of Transgenic Drosophila

      • Miki Fujioka, James B. Jaynes, Amy Bejsovec, Michael Weir

      Pages 353-363

   9. Transgenic Manipulation of the Sea Urchin Embryo

      • Jonathan P. Rast

      Pages 365-373

Developmental biology is one of the most exciting and fast-growing fields today. In part, this is so because the subject matter deals with the innately fascinating biological events—changes in form, structure, and function of the org- ism. The other reason for much of the excitement in developmental biology is that the field has truly become the unifying melting pot of biology, and provides a framework that integrates anatomy, physiology, genetics, biochemistry, and cellular and mole- lar biology, as well as evolutionary biology. No longer is the study of embryonic development merely “embryology.” In fact, development biology has produced - portant paradigms for both basic and clinical biomedical sciences alike. Although modern developmental biology has its roots in “experimental emb- ology” and the even more classical “chemical embryology,” the recent explosive and remarkable advances in developmental biology are critically linked to the advent of the “cellular and molecular biology revolution.” The impressive arsenal of expe- mental and analytical tools derived from cell and molecular biology, which promise to continue to expand, together with the exponentially developing sophistication in fu- tional imaging and information technologies, guarantee that the study of the devel- ing embryo will contribute one of the most captivating areas of biological research in the next millennium.

• Drosophila
• Neurulation
• Regeneration
• Vertebrate
• developmental biology
• evolution
• evolutionary biology
• genes
• physiology

"We live in a time of rising expectations. Being a developmental biologist at the turn of the millenium demands expertise in embryological manipulation, viral gene expression, in situ hybridization, videomicroscopy, in vitro assays, transgenic embryo production, cell lineage analysis, PCR techniques, and computer-enhanced imaging technology. Rocky Tuan and Cecilia Lo have done the field (and each scientist within it) a wonderful service by collecting and editing the protocols of the masters of each echnique. More than 100 papers spanning these areas (and more) patiently lead one through each method, giving details on what brand of microscope slides to use, what centrifuge tubes work best, and where to purchase each piece of equipment. Copious notes provide details based on the laboratories' experiences of what works and what doesn't. Developmental Biology Protocols is a library in itself and will be essential for every laboratory of developmental biology. This
is the collection to get before your next grant application is due."-Scott Gilbert, Department of Biology, Swarthmore College, Swarthmore, PA

"The range of techniques, from viral vectors to imaging, and of organisms, from sea urchins to mammals, is comprehensive yet focused. This work should be an invaluable aid to those interested in all aspects of comparative development. This work is logically organized and presents a great opportunity for not only picking up a technique but also placing it in a logical context. Well done!" -Barbara B. Knowles, Director of Research, Jackson Labs, Bar Harbor, ME

"Among similar laboratory manuals Developmental Biology Protocols edited by Tuan and Lo impress by their breadth of coverage, timeliness, and scrupulous attention to detail. . .these protocols will be an invaluable aid to every geneticist and developmental biologist."-Davor Solter, Director of Developmental Biology, Max-Planck Institute, Freiburg, Germany

• Thomas Jefferson University, Philadelphia

  Rocky S. Tuan

• University of Pennsylvania, Philadelphia

  Cecilia W. Lo

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