Developmental Biology Protocols: Volume II

  • Rocky S. Tuan
  • Cecilia W. Lo

Part of the Methods in Molecular Biology™ book series (MIMB, volume 136)

Table of contents

  1. Front Matter
    Pages i-xxviii
  2. Introduction

    1. Front Matter
      Pages 1-4
    2. Rocky S. Tuan, Cecilia W. Lo
      Pages 3-4
  3. Organogenesis

    1. Front Matter
      Pages 5-121
    2. Makoto Asashima, Takashi Ariizumi, Shuji Takahashi, George M. Malacinski
      Pages 15-26
    3. Calvin D. Roskelley, Colleen Wu, Aruna M. Somasiri
      Pages 27-38
    4. Harold Slavkin, Glen Nuckolls, Lillian Shum
      Pages 45-54
    5. Stefan Mundlos
      Pages 61-70
    6. Lisa M. Spain, Lisa L. Lau, Yousuke Takahama
      Pages 79-86
    7. Johannes A. A. Spijkers, Theodorus B. M. Hakvoort, Wouter H. Lamers
      Pages 87-100
    8. Cheng-Ming Chuong
      Pages 101-106
    9. Lin Lin, Zahra F. Zakeri
      Pages 107-113
  4. Abnormal Development and Teratology

    1. Front Matter
      Pages 123-281
    2. Gary C. Schoenwolf, Jodi L. Smith
      Pages 125-134
    3. Andrew Copp, Patricia Cogram, Angeleen Fleming, Dianne Gerrelli, Deborah Henderson, Andrew Hynes et al.
      Pages 135-160
    4. Takayuki Inagaki, Jodi L. Smith, Marion L. Walker, Gary C. Schoenwolf
      Pages 161-166
    5. Mary E. Desmond, Marcia C. Field
      Pages 179-184
    6. Barbara D. Abbott
      Pages 195-201
    7. Gary A. Held, Barbara D. Abbott
      Pages 203-217
    8. Sheila M. Bell, Claire M. Schreiner, William J. Scott
      Pages 219-226
    9. Masahiko Fujinaga
      Pages 227-238
    10. Andy Wessels, Roger Markwald
      Pages 239-259
    11. Christopher Lau, R. Woodrow Setzer
      Pages 271-281
  5. Screening and Mapping of Novel Genes and Mutations

    1. Front Matter
      Pages 283-495
    2. Lin Xu, Yefu Li
      Pages 285-296
    3. Gary E. Lyons, Bradley J. Swanson, Melissa A. Haendel, Joshua Daniels
      Pages 297-307
    4. Keith E. Latham, Elena De La Casa, Richard M. Schultz
      Pages 315-331
    5. Thomas B. Knudsen
      Pages 345-349
    6. Miki Fujioka, James B. Jaynes, Amy Bejsovec, Michael Weir
      Pages 353-363
    7. Jonathan P. Rast
      Pages 365-373
    8. Shuo Lin
      Pages 375-383
    9. Kristin L. Woods, Scott Schau, Mary Ellen Clark, Jacqueline A. Bonselaar, Robert J. Etches
      Pages 385-389
    10. Laura D. Zajchowski, S. Mansoor Mohammed, Qingxia Wei, Robert J. Etches
      Pages 391-397
    11. Keith E. Latham, Mark E. Westhusin
      Pages 405-425
    12. Jean Richa
      Pages 427-434
    13. Aya Jakobovits, Bruce T. Lamb, Kenneth R. Peterson
      Pages 435-453
    14. Siew-Sim Cheah, Richard R. Behringer
      Pages 455-463
    15. Jaspal S. Khillan
      Pages 465-476
    16. Yunzheng Le, Brian Sauer
      Pages 477-485

About this book


The molecular biology revolution has transformed developmental biology into one of the most exciting and fruitful fields in experimental biomedical research today. In Developmental Biology Protocols, established leaders in this field demonstrate this achievement with a comprehensive collection of cutting-edge protocols for studying and analyzing the events of embryonic development. Drawing on state-of-the-art cellular and molecular techniques, as well as new and sophisticated imaging and information technologies, this 2nd volume of three describes state-of-the-art methods for the study of organogenesis, the analysis of abnormal development and teratology, the screening and mapping of novel genes and mutations, and the application of transgenesis, including the production of transgenic animals and gene knockouts. The 1st and 3rd volumes in this seminal set complete today's widest-ranging collection of techniques designed to decipher the exact cellular, molecular, and genetic mechanisms that control the form, structure, and function of the developing embryo. Volume 1 presents readily reproducible methods for establishing and characterizing several widely used experimental model systems, for both the study of developmental patterns and morphogenesis, and the examination of embryo structure and function. In addition, there are step-by-step methods for the analysis of cell lineage, the production and use of chimeras, and the experimental molecular manipulation of embryos, including the application of viral vectors. No less innovative, volume 3 introduces powerful techniques for the manipulation of developmental gene expression and function, the analysis of gene expression, the characterization of tissue morphogenesis and development, the in vitro study of differentiation and development, and the genetic analysis of developmental models of diseases.
Highly practical and richly annotated, the three volumes of Developmental Biology Protocols describe multiple experimental systems and details techniques adopted from the broadest array of biomedical disciplines. Every researcher will not only better understand the principles, background, and rationale for how form and function are elaborated in an organism, but also gain full practical access to today's best methods for its analysis.


Drosophila Neurulation Regeneration Vertebrate developmental biology evolution evolutionary biology genes physiology

Editors and affiliations

  • Rocky S. Tuan
    • 1
  • Cecilia W. Lo
    • 2
  1. 1.Thomas Jefferson UniversityPhiladelphia
  2. 2.University of PennsylvaniaPhiladelphia

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2000
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-853-0
  • Online ISBN 978-1-59259-065-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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