Antigen Processing and Presentation Protocols

  • Joyce C. Solheim

Part of the Methods in Molecular Biology book series (MIMB, volume 156)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Jill R. Beyette, Timothy Hubbell, John J. Monaco
    Pages 1-16
  3. E. John Wherry, Deepa Rajagopal, Laurence C. Eisenlohr
    Pages 89-109
  4. Mary Ellexson-Turner, David Sidebottom, Sean Turner, Tara Bennett, William H. Hildebrand
    Pages 129-142
  5. Vashti G. Lacaille, Matthew J. Androlewicz
    Pages 143-151
  6. James Stevens, Geoffrey W. Butcher
    Pages 187-199
  7. Florence Boisgérault, Natalie Anosova, Eugenia V. Fedoseyeva, Robert C. Tam, Gilles Benichou
    Pages 211-218
  8. Francesca Galbiati, Luciano Adorini
    Pages 233-244
  9. Weisan Chen, Jack R. Bennink, Jonathan W. Yewde
    Pages 245-254
  10. Lisa M. Mendoza, Subramaniam Malarkannan, Nilabh Shastri
    Pages 255-263
  11. Subramaniam Malarkannan, Lisa M. Mendoza, Nilabh Shastri
    Pages 265-272
  12. Back Matter
    Pages 273-281

About this book


The processing and presentation of antigens by major histocompatibility complex (MHC) molecules is a crucial immunological phenomenon in infectious disease, malignancy, autoimmune disease, and transplantation. In Antigen Processing and Presentation Protocols, well-recognized and innovative experimentalists detail their cutting-edge methods for studying this complex process. Drawing on expertise from biochemistry, cell biology, and immunology, they describe step-by-step methods designed to question how MHC-binding peptides are generated, to test how peptides are delivered to MHC molecules, to analyze MHC peptide-binding patterns, and to assay the T cell response to the MHC/peptide complex. Each method is presented in sufficient detail to be readily reproducible and includes notes about potential pitfalls and tips on how to avoid failures. Emphasis is given those technical steps critical for experimental success that are often omitted from methods published in the primary literature.
Eminently accessible and state-of-the-art, Antigen Processing and Presentation Protocols provides both new and experienced investigators with highly practical tools that will extend the questions that can be asked, and effectively be answered, concerning antigen processing/presentation.

Editors and affiliations

  • Joyce C. Solheim
    • 1
  1. 1.University of Nebraska Medical CenterOmaha

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2001
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-745-8
  • Online ISBN 978-1-59259-062-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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