Chaperonin Protocols

  • Christine Schneider

Part of the Methods in Molecular Biology™ book series (MIMB, volume 140)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Elsie Quaite-Randall, Andrzej Joachimiak
    Pages 1-14
  3. Elsie Quaite-Randall, Andrzej Joachimiak
    Pages 15-27
  4. Elsie Quaite-Randall, Andrzej Joachimiak
    Pages 29-39
  5. Elsie Quaite-Randall, Andrzej Joachimiak
    Pages 41-49
  6. Saskia M. van der Vies, Peter A. Lund
    Pages 75-96
  7. Frank Weber, Manajit Hayer-Hartl
    Pages 111-115
  8. Manajit Hayer-Hartl
    Pages 127-132
  9. N. Kalaya Steede, Stacy L. Temkin, Samuel J. Landry
    Pages 133-138
  10. Yves Dubaquié, Renate Looser, Sabine Rospert
    Pages 139-143
  11. N. Kalaya Steede, Jesse J. Guidry, Samuel J. Landry
    Pages 145-151
  12. Raul G. Ferreyra, Judith Frydman
    Pages 153-160
  13. Vanita Thulasiraman, Raul G. Ferreyra, Judith Frydman
    Pages 161-167
  14. Vanitha Thulasiraman, Raul G. Ferreyra, Judith Frydman
    Pages 169-177
  15. Sally A. Lewis, Nicholas J. Cowan
    Pages 179-184
  16. Katja Siegers, Elmar Schiebel
    Pages 185-193
  17. Back Matter
    Pages 207-212

About this book


In Chaperonin Protocols, Christine Schneider has assembled a unique collection of readily reproducible protocols for the study of chaperonins, intracellular proteins critical to many biological processes. Written by experienced investigators who have successfully honed their methods to a fineness, the protocols focus on the purification of chaperonins from different species along with their corresponding cofactors, and on chaperonin activity assays for in vivo as well as in vitro work. Many activity assays are given for GroEL, which can also be applied to mitochrondrial Hsp60. There are also assays for the eukaryotic chaperonin TRiC and handy methods-for example, one for preparing labeled probes-that can be used for various purposes and prove helpful in numerous different procedures.
Critically important to a greater understanding of such disorders as cystic fibrosis, Alzheimer's disease, and BSE, Chaperonin Protocols offers both novice and experienced investigators fast access to today's best and most productive chaperonin methods, all explained in step-by-step detail to ensure robust and reproducible results.

Editors and affiliations

  • Christine Schneider
    • 1
  1. 1.Max-Planck Institute for BiochemistryMartinsriedGermany

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2000
  • Publisher Name Springer, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-739-7
  • Online ISBN 978-1-59259-061-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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