Capillary Electrophoresis of Nucleic Acids

Volume I: Introduction to the Capillary Electrophoresis of Nucleic Acids

  • Keith R. Mitchelson
  • Jing Cheng

Part of the Methods in Molecular Biology book series (MIMB, volume 162)

Table of contents

  1. Front Matter
    Pages i-xx
  2. Analytical Considerations for Capillary Electrophoresis

    1. Front Matter
      Pages 1-1
    2. Gary W. Slater, Claude Desruisseaux, Sylvain J. Hubert
      Pages 27-41
    3. Bruce McCord
      Pages 51-66
    4. Wenzhang Xie, Rong Yang, Junquan Xu, Liang Zhang, Wanli Xing, Jing Cheng
      Pages 67-83
    5. Norman J. Dovichi, JianZhong Zhang
      Pages 85-94
  3. Capillary Matrix: Considerations for Capillary Electrophoresis

    1. Front Matter
      Pages 109-109
    2. Marcella Chiari, Marina Cretich
      Pages 125-138
    3. Mark A. Quesada, Steve Menchen
      Pages 139-166
    4. Tim Wehr, Mingde Zhu, David T. Mao
      Pages 167-187
  4. Electrophoresis Matrix: Applications for DNA Fragment Sizing

    1. Front Matter
      Pages 201-201
    2. Benjamin Chu, Tianbo Liu, Chunhung Wu, Dehai Liang
      Pages 225-238
    3. Karel Klepárník, Zdeňvka Malá, Petr Bočvek
      Pages 239-258
    4. Keith R. Mitchelson, Jing Cheng
      Pages 259-277
  5. Practical Applications for Large DNA Fragment Sizing and Fragment Collection

    1. Front Matter
      Pages 291-291
    2. Christoph Heller, Soffia Magnúsdóttir, Jean-Louis Viovy
      Pages 293-305
    3. Michael D. Morris, Jeffrey J. Schwinefus, Olivia de Carmejane
      Pages 307-321
    4. Soffia Magnúsdóttir, Christoph Heller, Philippe Sergot, Jean-Louis Viovy
      Pages 323-331
    5. David T. Mao, Roy M. A. Lautamo
      Pages 333-344
  6. PracticalApplications for Oligonucleotide and Nucleotide Analysis

    1. Front Matter
      Pages 345-345
    2. Martin J. Pearce, Nigel D. Watson
      Pages 347-352
    3. G. Susan Srivatsa, Roya Pourmand, Steven Winters
      Pages 371-376
    4. Dan-Ke Xu, Hong-Yuan Chen
      Pages 377-382
  7. Practical Applications for Analysis of Environmental Damage and Cellular Metabolism of DNA

    1. Front Matter
      Pages 393-393
    2. Lisa A. Marzilli, Cheryl Koertje, Paul Vouros
      Pages 395-406
    3. Zeena E. Nackerdien, Barbara A. Siles, Stacey A. Nevins, Donald H. Atha
      Pages 407-417
    4. James Z. Xing, Trevor Carnelley, Jane Lee, William P. Watson, Ewan Booth, Michael Weinfeld et al.
      Pages 419-428
    5. Dieter L. Deforce, Elfride G. Van den Eeckhout
      Pages 429-441
    6. Guowang Xu, Hartmut M. Liebich, Rainer Lehmann, Sylvia Müller-Hagedorn
      Pages 459-474
  8. Back Matter
    Pages 475-484

About this book


The advent of capillary electrophoresis (CE) , with its power to separate and analyze very small amounts of DNA, has generated a host of DNA analytical procedures. In these novel and wide-ranging volumes of Capillary Electrophoresis of Nucleic Acids, an outstanding panel of hands-on experts and developers of CE equipment describe in step-by-step fashion their best cutting-edge methods for the detection and analysis of DNA mutations and modifications, ranging from precise DNA loci to entire genomes of organisms. This first volume of the set, Introduction to the Capillary Electrophoresis of Nucleic Acids, covers the practical and theoretical considerations behind the use of capillary electrophoresis for the analysis of small oligonucleotides and modified nucleotides. Along with detailed instructions ensuring ready reproducibility, these protocols offer time-tested advice on instrumentation, signal detection, the capillary environment, and the integration of mass spectrometry with CE. Several chapters are devoted to the analysis of small therapeutic oligonucleotides, nucleosides, and ribonucleotides by CE. The companion volume, Practical Applications of Capillary Electrophoresis, addresses techniques for high-throughput analysis of DNA fragments using SNP detection, mutation detection, DNA sequencing methods, and DNA-ligand interactions.
Comprehensive and up-to-date, the paired volumes of Capillary Electrophoresis of Nucleic Acids offer an authoritative guide with easy access to fast, versatile, reliable, and powerful technologies for all those basic and clinical investigators analyzing DNA variation today.

Editors and affiliations

  • Keith R. Mitchelson
    • 1
    • 2
  • Jing Cheng
    • 3
    • 4
  1. 1.Australian Genome Research FacilityUniversity of QueenslandBrisbane
  2. 2.Walter & Eliza Hall InstituteParkvilleAustralia
  3. 3.Biochip Research and Development Center, State Key Laboratory for Biomembrane and Membrane BiotechnologyTsinghua UniversityBeijingChina
  4. 4.Aviva Biosciences CorporationSan Diego

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2001
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-779-3
  • Online ISBN 978-1-59259-055-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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