Cytoskeleton Methods and Protocols

  • Ray H. Gavin

Part of the Methods in Molecular Biology™ book series (MIMB, volume 161)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Identification of Cytoskeleton Proteins

    1. Front Matter
      Pages 1-1
    2. Gangadhara Sailaja, Leslie M. Lincoln, Jifan Chen, David J. Asai
      Pages 17-27
  3. Microscopy Applications

    1. Front Matter
      Pages 29-29
    2. David R. Soil, Deborah Wessels, Edward Voss, Olof Johnson
      Pages 45-58
    3. Peter S. Walmod, Rasmus Hartmann-Petersen, Anton Berezin, Søren Prag, Vladislav V. Kiselyov, Vladimir Berezin et al.
      Pages 59-83
    4. Eugene A. Lepekhin, Peter S. Walmod, Anton Berezin, Vladimir Berezin, Elisabeth Bock
      Pages 85-100
    5. Uffe Koppelhus, Per Hellung-Larsen, Vagn Leick
      Pages 101-106
  4. Reagents for Studying Cytoskeleton Protein Function

    1. Front Matter
      Pages 107-107
    2. Andreas Holzinger
      Pages 109-120
  5. Cytoskeleton Dynamics

    1. Front Matter
      Pages 149-149
    2. Yisang Yoon, Kelly R. Pitts, Mark A. McNiven
      Pages 151-163
    3. Hong Cao, Heather Thompson, Eugene W. Krueger, Mark McNiven
      Pages 165-187
  6. Cellular Systems as Tools for Investigating Cytoskeleton Structure and Function

    1. Front Matter
      Pages 199-199
    2. Bruce R. Telzerand, Leah T. Haimo
      Pages 201-214
    3. Andrew W. Suddith, Eugeni A. Vaisberg, Sergei A. Kuznetsov, Walter Steffen, Conly L. Rieder, Robert E. Palazzo
      Pages 215-228
    4. Craig A. Mandato, * Kari L. Weber, * Anna J. Zandy, Thomas J. Keating, William M. Bement
      Pages 229-239
    5. Charles B. Lindemann, Kathleen A. Schmitz
      Pages 241-252
  7. Genetic Approaches for Studies of Cytoskeleton Protein Function

    1. Front Matter
      Pages 253-253
    2. David J. Asai, K. Mark, Leslie M. Lincoln, Renotta K. Smith
      Pages 269-278
  8. Back Matter
    Pages 285-288

About this book


Many recent advances in experimental instrumentation, reagents, and imaging technology have dramatically expanded the range of tools available for the study of the cytoskeleton. In Cytoskeleton Methods and Protocols, Ray Gavin brings together an international panel of experienced researchers to detail the readily reproducible methods that utilize biochemistry, immunology, genetics, microscopy, and image analysis for investigating cytoskeleton structure and function. Each protocol contains proven step-by-step instructions that enable both the novice and experienced researcher to achieve successful experimental results. The protocols utilize diverse model systems in a variety of organisms, including Saccharomyces, Micrasterias, Tetrahymena, Drosophila, Spisula, and Xenopus. Microscopy applications include digital-video microscopy and computer-assisted systems for the evaluation of cell motility and morphology. Help notes and tips accompany each protocol and provide additional, often unpublished, information that can make the difference between success and failure.
State-of-the-art and highly practical, Cytoskeleton Methods and Protocols makes available a diverse collection of powerful experimental systems and tools for successfully studying cytoskeleton structure and function.

Editors and affiliations

  • Ray H. Gavin
    • 1
  1. 1.Department of BiologyBrooklyn College, The City University of New York

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2001
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-771-7
  • Online ISBN 978-1-59259-051-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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