Overview
- Editors:
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John S. Elce
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Department of Biochemistry, Queen’s University, Kingston, Canada
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Table of contents (37 protocols)
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Assays of Calpain
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- Rodney P. Guttmann, Gail V. W. Johnson
Pages 143-150
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- Sucheta Kulkarni, Joan E. B. Fox
Pages 151-159
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Specific Tissues
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Front Matter
Pages 173-285
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- Patrick Cottin, Sylvie Poussard, Elise Dargelos, Denis Balcerzak, Bernadette Aragon, Jean Jacques Brustis et al.
Pages 173-180
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- Nechama S. Kosower, Sivia Barnoy
Pages 181-194
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- Naren L. Banik, Donald C. Shields
Pages 195-201
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- Xiaoning Bi, Ruifen Bi, Michel Baudry
Pages 203-217
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- Jennifer K. Newcomb, Brian R. Pike, Xiurong Zhao, Ronald L. Hayes
Pages 219-223
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- Barry G. Rosser, Gregory J. Gores
Pages 245-259
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- David Sindram, Pierre-Alain Clavien
Pages 261-266
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- Thomas R. Shearer, Hong Ma, Marjorie Shih, Chiho Fukiage, Mitsuyoshi Azuma
Pages 277-285
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Specific Substrates
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Front Matter
Pages 289-337
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About this book
The purpose of Calpain Methods and Protocols is quite straightf- ward: it is to present the actual experimental methods used in many different laboratories for the study of calpain. It will provide the vital experimental detail, and the discussion of possible pitfalls, for which the standard journals no longer provide space. This will make it as easy as possible for investi- tors interested in calpain to adopt established methods without repeating old mistakes, and to adapt and apply these methods in novel approaches to the many outstanding calpain questions. These questions range from purely biochemical problems of protein structure and enzyme regulation at the molecular level, through large areas of cell biology, to applied and clinical aspects of calpain function in human d- ease. Within this panoply of topics, a wide range of investigators will find many fascinating and as yet unanswered questions about calpain. Calpain Methods and Protocols will provide instant access to many essential te- niques, while saving them the time and effort involved in developing a new method. In addition to questions relating to the normal physiological roles of the calpains, there is considerable evidence that inappropriate calpain activity may have pathological effects in many tissues, for example, following ischemia. This provides a major stimulus for the development of specific calpain inhi- tors for therapeutic purposes, and for the development of methods to evaluate such inhibitors.
Reviews
"This book collects contributions from essentially all leading specialists in the area and the chapters are constructed in a way that should make it a useful bench-top companion. The book is good value for money: 36 chapters for $89 should make it an all but mandatory acquisition for libraries, and especially laboratories having programs on cell biology and cell pathology." -Cell Biology International
Editors and Affiliations
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Department of Biochemistry, Queen’s University, Kingston, Canada
John S. Elce