The Elisa Guidebook

  • John R. Crowther

Part of the Methods in Molecular Biology™ book series (MIMB, volume 149)

Table of contents

  1. Front Matter
    Pages i-xi
  2. John R. Crowther
    Pages 9-44
  3. John R. Crowther
    Pages 45-82
  4. John R. Crowther
    Pages 83-113
  5. John R. Crowther
    Pages 115-152
  6. John R. Crowther
    Pages 153-231
  7. John R. Crowther
    Pages 233-299
  8. John R. Crowther
    Pages 347-394
  9. John R. Crowther
    Pages 395-405
  10. John R. Crowther
    Pages 407-413
  11. Back Matter
    Pages 415-421

About this book


Highly sensitive, precise, and versatile, Enzyme Linked Immunosorbent Assay (ELISA) is now the predominant assay used in biomedical and agricultural research, as well as in applied science. In The ELISA Guidebook, John R. Crowther provides todayís premier practical guide to the understanding and application of ELISA. Updating and greatly expanding his widely appreciated earlier publication, ELISA Theory and Practice (1995), this important work introduces such major new topics as checkerboard titrations, quality control of testing, kit production and control, novel monoclonal antibodies, validation of assays, statistical requirements for data examination, and epidemiological considerations. The author describes each method in great detail to ensure experimental success and includes advice on equipment choice, maintenance, and calibration. Wherever possible, helpful written explanations are provided along with copious diagrams.
With its numerous worked examples, detailed instructions, and extensive illustrations, The ELISA Guidebook offers a powerful synthesis of all the basic concepts and practical experimental details investigators need to understand, develop, and apply the new ELISA methodology successfully in day-to-day basic and clinical research.

Authors and affiliations

  • John R. Crowther
    • 1
  1. 1.The International Atomic Energy AgencyViennaAustria

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2000
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-950-6
  • Online ISBN 978-1-59259-049-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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