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© 2000

Amino Acid Analysis Protocols

  • Catherine Cooper
  • Nicolle Packer
  • Keith Williams
Book

Part of the Methods in Molecular Biology™ book series (MIMB, volume 159)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Margaret I. Tyler
    Pages 1-7
  3. Frank D. Macchi, Felicity J. Shen, Rodney G. Keck, Reed J. Harris
    Pages 9-30
  4. Petr Jandik, Christopher Pohl, Victor Barreto, Nebojsa Avdalovic
    Pages 63-85
  5. Noriko Shindo, Tsutomu Fujimura, Saiko Kazuno, Kimie Murayama
    Pages 87-100
  6. Hiroyuki Kataoka, Sayuri Matsumura, Shigeo Yamamoto, Masami Makita
    Pages 101-122
  7. Hiroyuki Kataoka, Norihisa Sakiyama, Yukizo Ueno, Kiyohiko Nakai, Masami Makita
    Pages 183-206
  8. Qingyi Chu, Michael Zeece
    Pages 227-235
  9. Back Matter
    Pages 255-265

About this book

Introduction

Amino acid analysis is widely used in biotechnology, biomedical, and food analysis laboratories. Amino Acid Analysis Protocols constitutes a major collection of these indispensable analytical techniques, both classic and cutting-edge, of high utility for answering specific biological questions. Common methods include those based on HPLC or gas chromatography separation and analysis after precolumn derivatization. New techniques based on capillary electrophoresis separation, high-performance anion exchange chromatography, and mass spectrometry are also presented. Since results depend heavily on the quality of the sample, most contributors have devoted a section to sample preparation, particularly to the collection and storage of bodily fluids. A new method for desalting samples prior to hydrolysis is also provided. Each method is described in step-by-step detail to ensure successful experimental results, and contains helpful notes on pitfalls to avoid, and variations that enable the methods to be used with different systems.
Up-to-date and highly practical, Amino Acid Analysis Protocols offers analytical and clinical chemists, as well as a broad range of biological and biomedical investigators, a rich compendium of laboratory tools for the productive analysis of both common and uncommon amino acids.

Editors and affiliations

  • Catherine Cooper
    • 1
  • Nicolle Packer
    • 1
  • Keith Williams
    • 1
  1. 1.Proteome Systems LtdNorth RydeAustralia

Bibliographic information

  • Book Title Amino Acid Analysis Protocols
  • Editors Catherine Cooper
  • Series Title Methods in Molecular Biology™
  • DOI https://doi.org/10.1385/1592590470
  • Copyright Information Humana Press 2000
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-0-89603-656-7
  • Softcover ISBN 978-1-61737-117-2
  • eBook ISBN 978-1-59259-047-6
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XI, 265
  • Number of Illustrations 0 b/w illustrations, 0 illustrations in colour
  • Topics Biochemistry, general
  • Buy this book on publisher's site
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Reviews

"Sufficient detail and reference material is provided to allow application by a chemist generally familiar with analytical methods. Choices among the various procedures described will likely be made on an empirical basis."-Doody's Health Sciences Book Review Journal

"...the book presents a timely and detailed methodological reference for a broad range of current techniques used in amino acid analysis. The authors are all experts in the field and the editors (especially Williams) have considerable experience in amino acid analysis. this volume will provide an indispensable tool in any laboratory carrying out amino acid analysis."- Today's Life Science

"...This is a major collection of analytical techniques devoted to the analysis of amino acids in biotechnology, food analysis, and biomedical research, as well as the amino acid content of patient samples in clinical laboratories...This book is highly recommended for both engineers and experienced analysts interested in amino acid analysis; both will benefit from the information contained in this collection. It might also be a helpful resource for those looking for a replacement for old, dedicated amino acid analyzers for physiological samples by methods based on newer technologies and alternative techniques." - Clinical Chemistry

"Up-to-date and highly practical, Amino Acid Analysis Protocols offers analytical and clinical chemists as well as a broad range of biological and biomedical investigators a richer compendium of laboratory tools for the productive analysis of both common and uncommon amino acids." - Journal of Chromatographic Science