Connexin Methods and Protocols

  • Roberto Bruzzone
  • Christian Giaume

Part of the Methods In Molecular Biology™ book series (MIMB, volume 154)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Tools to Study Connexins

    1. Front Matter
      Pages 1-1
    2. Magali Théveniau-Ruissy, Sébastien Alcoléa, Irè Marics, Daniel Gros, Antoon F. M. Moorman, Wouter H. Lamers
      Pages 1-32
    3. Irene Dunia, Michel Recouvreur, Pierre Nicolas, Nalin M. Kumar, Hans Bloemendal, E. Lucio Benedetti
      Pages 33-55
    4. Gina E. Sosinsky, Guy A. Perkins
      Pages 57-75
    5. Matthias M. Falk
      Pages 91-116
    6. Judy K. Van Slyke, Linda S. Musil
      Pages 117-134
    7. Dale W. Laird, Karen Jordan, Qing Shao
      Pages 135-142
    8. Jean X. Jiang
      Pages 159-174
    9. Colin R. Green, Lee yong Law, Jun Sheng Lin, David L. Becker
      Pages 175-185
    10. Dieter Manthey, Klaus Willecke
      Pages 187-199
  3. Assays For Function

    1. Front Matter
      Pages 201-201
    2. I. Martha Skerrett, Mary Merritt, Lan Zhou, Hui Zhu, FengLi Cao, Joseph F. Smith et al.
      Pages 225-249
    3. Gerhard Dahl, Arnold Pfahnl
      Pages 251-268
    4. Harold V. M. Van Rijen, Ronald Wilders, Martin B. Rook, Habo J. Jongsma
      Pages 269-292
    5. Jean Déléze, Bruno Delage, Olfa Hentati-Ksibi, Franck Verrecchia, Jean-Claude Hervé
      Pages 313-327

About this book


An essential element in direct cell-cell communication between virtually all cell types in vertebrates, connexins have been recently linked to an increasing number of human genetic diseases. In Connexin Methods and Protocols, well established investigators describe in detail their best techniques for studying the cellular and molecular biology of connexins, as well as their physiological properties. These forefront methods are specifically designed to investigate the life of connexins from biosynthesis to assembly into gap junction channels, and also include powerful assays to perform functional studies on these routes of cell-cell communication. To ensure experimental success, each time-tested technique contains detailed step-by-step instructions, as well as tips on how to avoid pitfalls. Also discussed are the many specific
theoretical aspects of connexin biology and physiology.
Cutting-edge and highly practical, Connexin Methods and Protocols offers laboratory researchers a comprehensive collection of proven methods for studying the role of connexins in intercellular communication and human genetic disorders, as well as finding promising new therapeutics for those disorders.

Editors and affiliations

  • Roberto Bruzzone
    • 1
  • Christian Giaume
    • 2
  1. 1.Institut PasteurParisFrance
  2. 2.Collége de FranceParisFrance

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2001
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-658-1
  • Online ISBN 978-1-59259-043-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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