© 2000

The Nucleic Acid Protocols Handbook

  • Ralph Rapley

Part of the Springer Protocols Handbooks book series (SPH)

Table of contents

  1. Front Matter
    Pages i-xxii
  2. Nucleic Acid Extraction

    1. Front Matter
      Pages 1-1
    2. Sian Bryant, David L. Manning
      Pages 9-11
    3. Justin Stacey, Peter G. Isaac
      Pages 13-16
    4. Shu-Hua Cheng, Brandon D. Moore, Jeffrey R. Seemann
      Pages 17-22
    5. Elisabeth Chachaty, Patrick Saulnier
      Pages 29-32
    6. Surapareddy Sreenivasaprasad
      Pages 37-45
    7. John Heptinstall
      Pages 47-52
    8. John Heptinstall, Ralph Rapley
      Pages 57-60
  3. Basic Separation and Analysis of DNA

    1. Front Matter
      Pages 61-61
    2. Duncan R. Smith
      Pages 63-66
    3. D. Ross Williams, Ralph Rapley
      Pages 67-70
    4. Rachel Hodge
      Pages 71-72
    5. Adrian J. Harwood
      Pages 73-75
    6. Ralph Rapley, Jane Davenport-Jones
      Pages 77-79
    7. John Maule
      Pages 81-104

About this book


The Nucleic Acid Protocols Handbook is today's most comprehensive up-to-date treasury of all the key molecular biology methods-ranging from DNA extraction to gene localization in situ-needed to function effectively in the modern laboratory. Each of the 120 highly successful techniques follows the format of the much acclaimed Methods in Molecular Biology™ series, providing an introduction to the scientific basis of each technique, a complete listing of all the materials and reagents needed, and clear step-by-step instructions to permit error-free execution. Included for each technique are notes about pitfalls to avoid, troubleshooting tips, alternate methods, and explanations of the reasons for certain steps-all key elements contributing significantly to success in the lab. The authord are well-experienced masters of the techniques described, often having perfected and optimized them in their own laboratories. In The Nucleic Acid Protocols Handbook, Ralph Rapley has brought together the widest-ranging, one-volume collection of classic and cutting-edge techniques for the successful isolation, analysis, and manipulation of nucleic acids. Eminently practical and reproducible, these proven methods will help both experienced researchers and those entering the field attain the laboratory mastery needed-not only to deepen understanding of biological processes at the molecular level, but also to begin realizing the biotechnological bounty so clearly in prospect.


chromosome DNA enzyme gene gene expression genes hybridization mitochondria nucleic acid PCR RNA Southern Blot tissue transcription translation

Editors and affiliations

  • Ralph Rapley
    • 1
  1. 1.University of HertfordshireHatfieldUK

Bibliographic information

  • Book Title The Nucleic Acid Protocols Handbook
  • Editors Ralph Rapley
  • Series Title Springer Protocols Handbooks
  • Series Abbreviated Title Springer Protocols Handbooks
  • DOI
  • Copyright Information Humana Press 2000
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Book Archive
  • Hardcover ISBN 978-0-89603-459-4
  • Softcover ISBN 978-0-89603-841-7
  • eBook ISBN 978-1-59259-038-4
  • Series ISSN 1949-2448
  • Series E-ISSN 1949-2456
  • Edition Number 1
  • Number of Pages DLXXII, 478
  • Number of Illustrations 0 b/w illustrations, 0 illustrations in colour
  • Topics Cell Biology
  • Buy this book on publisher's site
Industry Sectors
Health & Hospitals


"... the techniques are presented in minute details and in a "cookbook-standardized manner"... The personal notes inserted by each contributor provide assurance that the techniques would work in any laboratory... Having all of those protocols updated and tested in practice and bound in one volume will be attractive to many scientists who deal with molecular biology techniques."- Modern Pathology

"There were over 190 authors involved in writing the protocols, ...each protocol is presented in outline format an follows a common numbering scheme, making it easy to find relevant sections....Many of the protocols include tables of data or figures, including drawings or photographs of gels...One of the most useful sections in each protocol lists notes, which elucidate possible pitfalls, provide troubleshooting tips, or give alternate methods or explanations for steps. This very practical information makes successful duplication of the procedure much more likely than when attempting to follow the often less detailed or less flexible descriptions given in journal articles....this handbook would be extremely useful as a reference in most university libraries."-E-Streams

" covers practically all of the very important and large field of nucleic acid research. The volume summarizes, in 10 parts, recent advances in the isolation , analysis and manipulation of nucleic acids. ...The chapters were contributed by authors from leading laboratories in the field all over the world....The book provides the reader with a large set of ready-to use protocols needed to undertake complex molecular biology experimental project on a large spectrum of biological models involving isolation and analysis of nucleic acids. It is thus an almost indispensable handbook for anybody working in the field of molecular biology and genetics."-Folia Microbiologica

"...extremely useful as a reference in most university libraries." - E-Streams

"...comprehensive and detailed...this is an set of recipes for the genetic kitchen." -The Biologist