The Nucleic Acid Protocols Handbook

  • Ralph Rapley

Part of the Springer Protocols Handbooks book series (SPH)

Table of contents

  1. Front Matter
    Pages i-xxii
  2. Nucleic Acid Extraction

    1. Front Matter
      Pages 1-1
    2. Sian Bryant, David L. Manning
      Pages 9-11
    3. Justin Stacey, Peter G. Isaac
      Pages 13-16
    4. Shu-Hua Cheng, Brandon D. Moore, Jeffrey R. Seemann
      Pages 17-22
    5. Elisabeth Chachaty, Patrick Saulnier
      Pages 29-32
    6. Surapareddy Sreenivasaprasad
      Pages 37-45
    7. John Heptinstall
      Pages 47-52
    8. John Heptinstall, Ralph Rapley
      Pages 57-60
  3. Basic Separation and Analysis of DNA

    1. Front Matter
      Pages 61-61
    2. Duncan R. Smith
      Pages 63-66
    3. D. Ross Williams, Ralph Rapley
      Pages 67-70
    4. Rachel Hodge
      Pages 71-72
    5. Adrian J. Harwood
      Pages 73-75
    6. Ralph Rapley, Jane Davenport-Jones
      Pages 77-79
    7. John Maule
      Pages 81-104
    8. Elena D. Katz
      Pages 105-113
  4. Probe Design, Synthesis, and Labeling

    1. Front Matter
      Pages 115-115
    2. Adrian J. Harwood
      Pages 117-121
    3. Jane Davenport-Jones
      Pages 123-125
    4. Y. M. Dennis Lo, Shu F. An
      Pages 127-133
    5. Sue Fowler, Ian Durrant
      Pages 135-143
    6. Ian Durrant, Timothy Stone
      Pages 145-147
    7. Bronwen M. Harvey, Claire B. Wheeler, Martin W. Cunningham
      Pages 149-152
    8. Claire B. Wheeler, Bronwen M. Harvey, Martin Cunningham
      Pages 153-156
    9. Eric Quéméneur
      Pages 169-173
  5. RNA Analysis Techniques

    1. Front Matter
      Pages 175-175
    2. Sian Bryant, David L. Manning
      Pages 177-179
    3. Dominique Belin
      Pages 181-194
    4. Maggie Walmsley, Mark Leonard, Roger Patient
      Pages 195-199
    5. Stéphane Viville, Roberto Mantovani
      Pages 201-206
    6. Ulrich Pfeffer, Paola Ferro
      Pages 213-217
    7. Rainer Löw
      Pages 239-247
    8. Rai Ajit K. Srivastava
      Pages 249-257
  6. Gene Library Construction and Screening

    1. Front Matter
      Pages 259-259
    2. Kris N. Lambert, Valerie M. Williamson
      Pages 289-294
    3. Alan R. Shuldiner, Keith Tanner
      Pages 295-303
    4. Clifford W. Schweinfest, Peter S. Nelson, Michael W. Graber, Rita I. Demopoulos, Takis S. Papas
      Pages 305-318

About this book


The Nucleic Acid Protocols Handbook is today's most comprehensive up-to-date treasury of all the key molecular biology methods-ranging from DNA extraction to gene localization in situ-needed to function effectively in the modern laboratory. Each of the 120 highly successful techniques follows the format of the much acclaimed Methods in Molecular Biology™ series, providing an introduction to the scientific basis of each technique, a complete listing of all the materials and reagents needed, and clear step-by-step instructions to permit error-free execution. Included for each technique are notes about pitfalls to avoid, troubleshooting tips, alternate methods, and explanations of the reasons for certain steps-all key elements contributing significantly to success in the lab. The authord are well-experienced masters of the techniques described, often having perfected and optimized them in their own laboratories. In The Nucleic Acid Protocols Handbook, Ralph Rapley has brought together the widest-ranging, one-volume collection of classic and cutting-edge techniques for the successful isolation, analysis, and manipulation of nucleic acids. Eminently practical and reproducible, these proven methods will help both experienced researchers and those entering the field attain the laboratory mastery needed-not only to deepen understanding of biological processes at the molecular level, but also to begin realizing the biotechnological bounty so clearly in prospect.


chromosome DNA enzyme gene gene expression genes hybridization mitochondria nucleic acid PCR RNA Southern Blot tissue transcription translation

Editors and affiliations

  • Ralph Rapley
    • 1
  1. 1.University of HertfordshireHatfieldUK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2000
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Book Archive
  • Print ISBN 978-0-89603-459-4
  • Online ISBN 978-1-59259-038-4
  • Series Print ISSN 1949-2448
  • Series Online ISSN 1949-2456
  • Buy this book on publisher's site
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