Bioluminescence Methods and Protocols

  • Robert A. LaRossa

Part of the Methods in Molecular Biology™ book series (MIMB, volume 102)

Table of contents

  1. Front Matter
    Pages i-x
  2. The Basics

    1. Front Matter
      Pages 1-1
    2. Sharon R. Ford, Franklin R. Leach
      Pages 3-20
    3. Amy Cheng Vollmer
      Pages 21-31
    4. Steven R. Kain, Guohong Zhang, Vanessa Gurtu, Paul A. Kitts
      Pages 33-42
  3. Analytical Biochemistry

    1. Front Matter
      Pages 43-43
    2. Eberhard Jüngling, Helmut Kammermeier, Yvan Fischer
      Pages 45-53
    3. Sharon R. Ford, Franklin R. Leach
      Pages 55-68
    4. Sharon R. Ford, Franklin R. Leach
      Pages 69-81
  4. Molecular Biology Tools

    1. Front Matter
      Pages 83-83
    2. Tina K. Van Dyk, Reinhardt A. Rosson
      Pages 85-95
    3. David C. Alexander, Michael S. DuBow
      Pages 105-122
    4. L. Winona Wagner, Tina K. Van Dyk
      Pages 123-127
    5. Vanitha Thulasiraman, Robert L. Matts
      Pages 129-141
  5. Cell-Based Assays

    1. Front Matter
      Pages 143-143
    2. Amy Cheng Vollmer
      Pages 145-151
    3. Matti Korpela, Marko Virta, Matti Karp
      Pages 161-168
    4. Ian A. Cree
      Pages 169-177
    5. Ian A. Cree
      Pages 179-187
  6. Environmental Applications

    1. Front Matter
      Pages 199-199
    2. Michael A. Costanzo, Julie Guzzo, Michael S. DuBow
      Pages 201-217
    3. Marko Virta, Sisko Tauriainen, Matti Karp
      Pages 219-229
    4. Tamar Barkay, Ralph R Turner, Lasse D. Rasmussen, Carol A. Kelly, John W. M. Rudd
      Pages 231-246
    5. Annelie Möller, Janet K. Jansson
      Pages 269-283
    6. Riccardo Tombolini, Janet K. Jansson
      Pages 285-298
  7. Back Matter
    Pages 299-310

About this book


Comprehensive and state-of-the-art, Bioluminescence Methods and Protocols brings together a collection of innovative methods for the use of luciferase and green fluorescent protein in analytical biochemistry, molecular toxicology, environmental monitoring, and cell-based assays. The contributors-all hands-on researchers who have perfected their protcols-illustrate these powerful methods with a wide variety of applications ranging from the analytical determination (titer) of specific molecules to the cellular detection of a wide range of pollutants, to microbiological detection and analysis, and the molecular biological manipulation of lux, luc, and gfp structural genes. Step-by-step methods for the construction of other recombinant organisms are also included.

Bioluminescence Methods and Protocols represents the state-of-the-art in luminescent assays. Its cutting-edge techniques, diversely illustrated applications, and detailed instructions will make it the standard reference for today's investigators who want to adapt established techniques to their purposes or apply them to a wide range of clinical, microbiological, environmental, toxicological, and analytical problems.

Editors and affiliations

  • Robert A. LaRossa
    • 1
  1. 1.DuPont Co.Wilmington

Bibliographic information

  • DOI
  • Copyright Information Humana Press 1998
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-520-1
  • Online ISBN 978-1-59259-577-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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