Phospholipid Signaling Protocols

  • Ian M. Bird

Part of the Methods in Molecular Biology™ book series (MIMB, volume 105)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Monitoring of Activation of Phospholipid-Derived Cell Signaling Pathways

    1. Front Matter
      Pages xiii-xiii
    2. Wendy Bollinger Bollag, Richard D. Griner
      Pages 89-98
    3. Rachel E. Meyers, Lewis C. Cantley
      Pages 99-108
    4. Gregory J. Parker, Joost C. Loijens, Richard A. Anderson
      Pages 127-139
    5. Wendy Bollinger Bollag
      Pages 151-160
    6. Sandie I. Briand, Sylvie G. Bernier, Gaétan Guillemette
      Pages 161-166
    7. Jacob Vadakekalam, Stewart Metz
      Pages 175-185
    8. Bruno Battistini, Serge Picard, Pierre Borgeat, Pierre Sirois
      Pages 187-199
    9. Bruno Battistini, Serge Picard, Pierre Borgeat, Pierre Sirois
      Pages 201-207
    10. Pino Santana, C. M. de Ruiz Galarreta, Luisa F. Fanjul
      Pages 223-231
    11. Ana Olivera, Sarah Spiegel
      Pages 233-242
  3. General Methods for Monitoring Changes in Levels of Key Signaling Pathway Proteins and Associated mRNA

    1. Front Matter
      Pages 286-286
    2. Cynthia E. Shaw, Jing Zheng
      Pages 295-306
    3. Daniel S. Millican, Ian M. Bird
      Pages 315-324
    4. Daniel S. Millican, Ian M. Bird
      Pages 337-350
  4. Back Matter
    Pages 373-380

About this book


In Phospholipid Signaling Protocols, state-of-the-art techniques for monitoring the major lipid and phospholipid-derived second messenger pathways to identify and quantify pathway activation are detailed by experts intimately experienced in their use. The assays described cover all the major phospholipases (C, D, A2), as well as sphingomyelinase and its associated metabolites. Additional protocols are provided for the assay of phosphoinositide 3-, 4-, and 5-kinase and sphingosine kinase activity, and for the quantification, separation, and rigorous identification of phospholipids, diacylglycerol, and sphingolipids, as well as their metabolites, including phosphoinositols, choline metabolites, and fatty acid metabolites. In addition, there is extensive information on the extraction, size separation, detection, and quantification of cellular signaling proteins and corresponding mRNA, as well as a description of their localization by immunohistochemistry and immunocytochemistry.

Phospholipid Signaling Protocols offers a wide-ranging collection of cutting-edge techniques for the study of signal transduction through phospholipid intermediates and their metabolites. The book is an indispensable reference for both the newcomer and the experienced researcher seeking to expand knowledge of these critical pathways, and strongly complements its companion volumes-R.A.J. Challiss' Receptor Signal Transduction Protocols, D. Bar-Sagi's, Transmembrane Signaling Protocols, and D. A. Kendall and S.J. Hill's Signal Transduction Protocols-in building a unique library of tried-and-tested protocols relating to the ever expanding signal transduction field.

Editors and affiliations

  • Ian M. Bird
    • 1
  1. 1.University of Wisconsin at Madison

Bibliographic information

  • DOI
  • Copyright Information Humana Press 1998
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-491-4
  • Online ISBN 978-1-59259-255-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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