Recombinant Protein Protocols

Detection and Isolation

  • Rocky S. Tuan

Part of the Methods in Molecular Biology™ book series (MIMB, volume 63)

Table of contents

  1. Front Matter
    Pages i-xx
  2. Introduction

  3. Detection and Isolation of Expressed Proteins

    1. Debyra Groskreutz, Elaine T. Schenborn
      Pages 11-30
    2. Corinne E. M. Olesen, John J. Fortin, John C. Voyta, Irena Bronstein
      Pages 61-70
    3. Takeshi Sano, Cassandra L. Smith, Charles R. Cantor
      Pages 119-128
    4. Li Zhu
      Pages 173-196
    5. Erica A. Golemis, Vladimir Khazak
      Pages 197-218
  4. Detection of Recombinant Gene Expression In Situ

    1. Ruth Sullivan, Cecilia W. Lo
      Pages 229-246
    2. Ronald A. Conlon
      Pages 271-274
    3. Omar Bagasra, Muhammad Mukhtar, Farida Shaheen, Roger J. Pomerantz
      Pages 275-303
    4. Steven R. Kain, Paul Kitts
      Pages 305-324
  5. Applications of Recombinant Gene Expression

    1. Henry Daniell, Chittibabu Guda, David T. McPherson, Xiaorong Zhang, Jie Xu, Dan W. Urry
      Pages 359-371
    2. Victoria E. Carr-Brendel, Robin L. Geller, Tracy J. Thomas, Daniel R. Boggs, Susan K. Young, JoAnne Crudele et al.
      Pages 373-387
    3. Georg Sczakiel, Giorgio Palú, William James
      Pages 389-400
    4. Yaolin Wang, Bert W. O’Malley, Sophia Y. Tsai
      Pages 401-413
    5. Muhammad Mukhtar, Zahida Parveen, Omar Bagasra
      Pages 415-437
  6. Back Matter
    Pages 439-448

About this book


A major success story of modem molecular biology is the development of technologies to clone and express specific genes. Current applications of recombinant gene products cover a wide spectrum, including gene therapy, production of bioactive pharmaceuticals, synthesis of novel biopolymers, agriculture and animal husbandry, and so on. Inherent in bringing these appli­ cations to fruition is the need to design "expression constructs" that will per­ mit the ready and specific detection and isolation of the defined recombinant gene products. Recombinant Protein Protocols grows out of the need for a laboratory manual on the detection and isolation of recombinantly expressed genes that covers both the background information and the practical laboratory recipes for these analyses. In this book, detailed and contemporary protocols are col­ lected to provide the reader with a wide-ranging number of methodologies to enhance the detection and isolation of their gene product(s) of interest. A large number of molecular tags and labels and their usage are described, including enzymes, ligand-binding moieties, immunodetectable molecules, as well as methods to detect interactive proteins, and gene expression-mediated alter­ ations in cellular activity. Chapters on in situ detection of gene expression deal with technologies that are currently being applied to the study of gene function and activity. Highlights of applications for recombinant gene expres­ sion technologies are provided to give readers exciting perspectives on the future of such technologies.

Editors and affiliations

  • Rocky S. Tuan
    • 1
  1. 1.Thomas Jefferson UniversityPhiladelphia

Bibliographic information

  • DOI
  • Copyright Information Humana Press 1997
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-481-5
  • Online ISBN 978-1-59259-549-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
Industry Sectors
Health & Hospitals