Arabidopsis Protocols

  • José M. Martinez-Zapater
  • Julio Salinas

Part of the Methods in Molecular Biology™ book series (MIMB, volume 82)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Arabidopsis Culture

    1. Front Matter
      Pages 1-1
    2. Randy Scholl, Luz Rivero-Lepickas, Deborah Crist
      Pages 1-12
    3. Peter McCourt, Kallie Keith
      Pages 13-17
    4. Jaideep Mathur, Csaba Koncz
      Pages 27-30
    5. Jaideep Mathur, Csaba Koncz
      Pages 31-34
    6. Jaideep Mathur, Csaba Koncz
      Pages 35-42
  3. Purification of Subcellular Organelles and Macromolecules

    1. Front Matter
      Pages 41-41
    2. Mathieu Klein, Stefan Binder, Axel Brennicke
      Pages 49-53
    3. Jianming Li, Joanne Chory
      Pages 55-60
    4. David Bouchez, Christine Camilleri
      Pages 61-70
    5. George S. Mourad
      Pages 71-77
    6. Mathieu Klein, Rudolf Hiesel, Charles Andre, Axel Brennicke
      Pages 79-84
    7. Clifford D. Carpenter, Anne E. Simon
      Pages 85-89
  4. Mutagenesis and Genetic Analysis

    1. Front Matter
      Pages 89-89
    2. Jonathan Lightner, Timothy Caspar
      Pages 91-102
    3. Maarten Koornneef, Carlos Alonso-Blanco, Piet Stam
      Pages 105-117
    4. John S. Heslop-Harrison
      Pages 119-127
    5. Rodney G. Winkler, Kenneth A. Feldmann
      Pages 129-136
  5. Gene Mapping in Arabidopsis

    1. Front Matter
      Pages 135-135
    2. Carlos Alonso-Blanco, Maarten Koornneef, Piet Stam
      Pages 137-146
    3. Pieter Vos
      Pages 147-155
    4. Jane Glazebrook, Eliana Drenkarci, Daphne Preuss, Frederick M. Ausubel
      Pages 173-182
    5. Anton R. Schiiffner
      Pages 183-198
    6. Francis D. Agyare, Gus Lagos, Deval Lashkari, Ronald W. Davis, Bertrand Lemieux
      Pages 199-208
  6. Transient and Stable Transformation

    1. Front Matter
      Pages 207-207
    2. Steffen Abel, Athanasios Theologis
      Pages 209-217
    3. Annette C. Vergunst, Ellen C. de Waal, Paul J. J. Hooykaas
      Pages 227-244
    4. Eric van der Graaff, Paul J. J. Hooykaas
      Pages 245-258
    5. Jaideep Mathur, Csaba Koncz
      Pages 267-276
  7. VI Gene Cloning Strategies

    1. Front Matter
      Pages 275-275
    2. Jeffrey Leung, Jéréme Giraudat
      Pages 277-303
    3. Ann Van Gysel, Gerda Cnops, Peter Breyne, Marc Van Montagu, Maria Teresa Cervera
      Pages 305-314
    4. Deborah Long, George Coupland
      Pages 315-328
    5. Andy Pereira, Mark G. M. Aarts
      Pages 329-338
    6. Brian P. Dilkes, Kenneth A. Feldmann
      Pages 339-351
  8. Gene Expression Analyses

    1. Front Matter
      Pages 351-351
    2. Gary N. Drews
      Pages 353-371
    3. Janice de Almeida Engler, Marc Van Montagu, Gilbert Engler
      Pages 373-384
    4. William H.R Langridge, Aladar A. Szalay
      Pages 385-396
    5. Qingzhong Kong, Anne E. Simon
      Pages 409-415
    6. Anna-Lisa Paul, Robert J. Ferl
      Pages 417-429
  9. Back Matter
    Pages 431-440

About this book


n Arabidopsis Protocols internationally recognized experts offer a comprehensive collection of significant new methods for gene mapping, cloning, and reverse genetics, as well as updated standard protocols for work in Arabidopsis genetics and molecular biology. These time-tested methods range from techniques for the successful growing of Arabidopsis to strategies for gene cloning. The sections on genetics, transformation, and gene expression analysis will be especially helpful to scientists involved in mutant analysis or in producing and analyzing transgenic plants. In many cases, the protocols can also be applied to other plant species, with minor adjustments.
Serving both experienced researchers and beginners in the field, Arabidopsis Protocols will immediately become the standard reference for laboratories working with Arabidopsis, the plant of choice to approach many fundamental questions in plant biology.

Editors and affiliations

  • José M. Martinez-Zapater
    • 1
  • Julio Salinas
    • 1
  1. 1.Centro de Investigación y TecnologiaInstituto Nacional de Investigación y Tecnologia Agraria y AlimentariaMadridSpain

Bibliographic information

  • DOI
  • Copyright Information Humana Press 1998
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-391-7
  • Online ISBN 978-1-59259-268-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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